Matches in SemOpenAlex for { <https://semopenalex.org/work/W1562589345> ?p ?o ?g. }
- W1562589345 endingPage "281" @default.
- W1562589345 startingPage "269" @default.
- W1562589345 abstract "Summary The conformational dynamism and aggregate state of small heat shock proteins (sHSPs) may be crucial for their functions in thermoprotection of plant cells from the detrimental effects of heat stress. Ectopic expression of single chain fragment variable (scFv) antibodies against cytosolic sHSPs was used as new tool to generate sHSP loss‐of‐function mutants by antibody‐mediated prevention of the sHSP assembly in vivo . Anti‐sHSP scFv antibodies transiently expressed in heat‐stressed tobacco protoplasts were not only able to recognize the endogenous sHSPs but also prevented their assembly into heat stress granula (HSGs). Constitutive expression of the same scFv antibodies in transgenic plants did not alter their phenotype at normal growth temperatures, but their leaves turned yellow and died after prolonged stress at sublethal temperatures. Structural analysis revealed a regular cytosolic distribution of stress‐induced sHSPs in mesophyll cells of stress‐treated transgenic plants, whereas extensive formation of HSGs was observed in control cells. After prolonged stress at sublethal temperatures, mesophyll cells of transgenic plants suffered destruction of all cellular membranes and finally underwent cell death. In contrast, mesophyll cells of the stressed controls showed HSG disintegration accompanied by appearance of polysomes, dictyosomes and rough endoplasmic reticulum indicating normalization of cell functions. Apparently, the ability of sHSPs to assemble into HSGs as well as the HSG disintegration is a prerequisite for survival of plant cells under continuous stress conditions at sublethal temperatures." @default.
- W1562589345 created "2016-06-24" @default.
- W1562589345 creator A5000626078 @default.
- W1562589345 creator A5004773712 @default.
- W1562589345 creator A5046846895 @default.
- W1562589345 creator A5047018283 @default.
- W1562589345 creator A5075072003 @default.
- W1562589345 creator A5079742060 @default.
- W1562589345 date "2004-12-02" @default.
- W1562589345 modified "2023-10-10" @default.
- W1562589345 title "Immunomodulation of function of small heat shock proteins prevents their assembly into heat stress granules and results in cell death at sublethal temperatures" @default.
- W1562589345 cites W1566588664 @default.
- W1562589345 cites W1790977108 @default.
- W1562589345 cites W1797354227 @default.
- W1562589345 cites W1797775581 @default.
- W1562589345 cites W1826004002 @default.
- W1562589345 cites W1864002683 @default.
- W1562589345 cites W1965876297 @default.
- W1562589345 cites W1966762686 @default.
- W1562589345 cites W1974553248 @default.
- W1562589345 cites W1975993749 @default.
- W1562589345 cites W1976770637 @default.
- W1562589345 cites W1981362268 @default.
- W1562589345 cites W1983445269 @default.
- W1562589345 cites W1985610916 @default.
- W1562589345 cites W1986123269 @default.
- W1562589345 cites W1986447265 @default.
- W1562589345 cites W1997678546 @default.
- W1562589345 cites W2009101312 @default.
- W1562589345 cites W2013288236 @default.
- W1562589345 cites W2023692919 @default.
- W1562589345 cites W2024556021 @default.
- W1562589345 cites W2026030247 @default.
- W1562589345 cites W2027231880 @default.
- W1562589345 cites W2027239762 @default.
- W1562589345 cites W2031185322 @default.
- W1562589345 cites W2035843908 @default.
- W1562589345 cites W2035851443 @default.
- W1562589345 cites W2036910883 @default.
- W1562589345 cites W2036973010 @default.
- W1562589345 cites W2039315146 @default.
- W1562589345 cites W2039480523 @default.
- W1562589345 cites W2045866234 @default.
- W1562589345 cites W2048890617 @default.
- W1562589345 cites W2055268010 @default.
- W1562589345 cites W2059418591 @default.
- W1562589345 cites W2066620645 @default.
- W1562589345 cites W2067056513 @default.
- W1562589345 cites W2076048646 @default.
- W1562589345 cites W2080582085 @default.
- W1562589345 cites W2080682710 @default.
- W1562589345 cites W2080877814 @default.
- W1562589345 cites W2081775218 @default.
- W1562589345 cites W2083333030 @default.
- W1562589345 cites W2084664870 @default.
- W1562589345 cites W2090702508 @default.
- W1562589345 cites W2109975826 @default.
- W1562589345 cites W2114587202 @default.
- W1562589345 cites W2115547367 @default.
- W1562589345 cites W2123582659 @default.
- W1562589345 cites W2123608704 @default.
- W1562589345 cites W2126428959 @default.
- W1562589345 cites W2127033103 @default.
- W1562589345 cites W2127682359 @default.
- W1562589345 cites W2129797991 @default.
- W1562589345 cites W2132489088 @default.
- W1562589345 cites W2138461857 @default.
- W1562589345 cites W2142233561 @default.
- W1562589345 cites W2145359681 @default.
- W1562589345 cites W2147961963 @default.
- W1562589345 cites W2148573587 @default.
- W1562589345 cites W2157149282 @default.
- W1562589345 cites W2164887975 @default.
- W1562589345 cites W2496024654 @default.
- W1562589345 cites W4240617312 @default.
- W1562589345 cites W4243642973 @default.
- W1562589345 cites W4293247451 @default.
- W1562589345 cites W4365786542 @default.
- W1562589345 cites W4376043815 @default.
- W1562589345 doi "https://doi.org/10.1111/j.1365-313x.2004.02290.x" @default.
- W1562589345 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/15634203" @default.
- W1562589345 hasPublicationYear "2004" @default.
- W1562589345 type Work @default.
- W1562589345 sameAs 1562589345 @default.
- W1562589345 citedByCount "64" @default.
- W1562589345 countsByYear W15625893452012 @default.
- W1562589345 countsByYear W15625893452013 @default.
- W1562589345 countsByYear W15625893452014 @default.
- W1562589345 countsByYear W15625893452015 @default.
- W1562589345 countsByYear W15625893452016 @default.
- W1562589345 countsByYear W15625893452017 @default.
- W1562589345 countsByYear W15625893452018 @default.
- W1562589345 countsByYear W15625893452019 @default.
- W1562589345 countsByYear W15625893452020 @default.
- W1562589345 countsByYear W15625893452021 @default.
- W1562589345 countsByYear W15625893452022 @default.
- W1562589345 countsByYear W15625893452023 @default.
- W1562589345 crossrefType "journal-article" @default.