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- W1562641260 abstract "Publisher Summary Centrosome kinases can be identified primarily through one of two routes—(1) through performing subcellular localization studies on previously characterized protein kinases and (2) through screens directed at identifying cell cycle defects in genetically tractable organisms. The genetic approaches or monoclonal antibody screens are not targeted at specific identification of protein kinases. The “candidate” approach of looking at the subcellular localization of a previously identified protein kinase has been the most productive method for the identification of novel centrosomal kinases in the near future. This chapter describes protocols that allow the unambiguous assignment of a previously characterized or newly isolated protein kinase to the centrosome. The chapter also discusses techniques that might soon become available to allow a systematic identification of centrosomal kinases, as well as indicating methods that address both their function and substrates." @default.
- W1562641260 created "2016-06-24" @default.
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- W1562641260 date "2001-01-01" @default.
- W1562641260 modified "2023-09-27" @default.
- W1562641260 title "Identification of centrosome kinases" @default.
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- W1562641260 doi "https://doi.org/10.1016/s0091-679x(01)67021-3" @default.
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