Matches in SemOpenAlex for { <https://semopenalex.org/work/W1562983132> ?p ?o ?g. }
- W1562983132 endingPage "12530" @default.
- W1562983132 startingPage "12513" @default.
- W1562983132 abstract "Real-time quantitative PCR (RT-qPCR) is a reliable and widely used method for gene expression analysis. The accuracy of the determination of a target gene expression level by RT-qPCR demands the use of appropriate reference genes to normalize the mRNA levels among different samples. However, suitable reference genes for RT-qPCR have not been identified in Sacha inchi (Plukenetia volubilis), a promising oilseed crop known for its polyunsaturated fatty acid (PUFA)-rich seeds. In this study, using RT-qPCR, twelve candidate reference genes were examined in seedlings and adult plants, during flower and seed development and for the entire growth cycle of Sacha inchi. Four statistical algorithms (delta cycle threshold (ΔCt), BestKeeper, geNorm, and NormFinder) were used to assess the expression stabilities of the candidate genes. The results showed that ubiquitin-conjugating enzyme (UCE), actin (ACT) and phospholipase A22 (PLA) were the most stable genes in Sacha inchi seedlings. For roots, stems, leaves, flowers, and seeds from adult plants, 30S ribosomal protein S13 (RPS13), cyclophilin (CYC) and elongation factor-1alpha (EF1α) were recommended as reference genes for RT-qPCR. During the development of reproductive organs, PLA, ACT and UCE were the optimal reference genes for flower development, whereas UCE, RPS13 and RNA polymerase II subunit (RPII) were optimal for seed development. Considering the entire growth cycle of Sacha inchi, UCE, ACT and EF1α were sufficient for the purpose of normalization. Our results provide useful guidelines for the selection of reliable reference genes for the normalization of RT-qPCR data for seedlings and adult plants, for reproductive organs, and for the entire growth cycle of Sacha inchi." @default.
- W1562983132 created "2016-06-24" @default.
- W1562983132 creator A5008166282 @default.
- W1562983132 creator A5011985227 @default.
- W1562983132 creator A5015538983 @default.
- W1562983132 creator A5020583473 @default.
- W1562983132 creator A5027148089 @default.
- W1562983132 creator A5037069151 @default.
- W1562983132 creator A5050739400 @default.
- W1562983132 creator A5068438174 @default.
- W1562983132 creator A5077466803 @default.
- W1562983132 date "2015-06-03" @default.
- W1562983132 modified "2023-10-13" @default.
- W1562983132 title "Selection of Reliable Reference Genes for Gene Expression Studies of a Promising Oilseed Crop, Plukenetia volubilis, by Real-Time Quantitative PCR" @default.
- W1562983132 cites W1534062799 @default.
- W1562983132 cites W1577577364 @default.
- W1562983132 cites W1967896481 @default.
- W1562983132 cites W1970425788 @default.
- W1562983132 cites W1972071470 @default.
- W1562983132 cites W1974517295 @default.
- W1562983132 cites W1982850386 @default.
- W1562983132 cites W1991814173 @default.
- W1562983132 cites W1994669247 @default.
- W1562983132 cites W2008183439 @default.
- W1562983132 cites W2013061140 @default.
- W1562983132 cites W2025492442 @default.
- W1562983132 cites W2031523141 @default.
- W1562983132 cites W2033499402 @default.
- W1562983132 cites W2033815454 @default.
- W1562983132 cites W2048865019 @default.
- W1562983132 cites W2058453385 @default.
- W1562983132 cites W2072571453 @default.
- W1562983132 cites W2076124940 @default.
- W1562983132 cites W2078586425 @default.
- W1562983132 cites W2080321548 @default.
- W1562983132 cites W2083274319 @default.
- W1562983132 cites W2083922459 @default.
- W1562983132 cites W2088774263 @default.
- W1562983132 cites W2089035529 @default.
- W1562983132 cites W2099824652 @default.
- W1562983132 cites W2105423509 @default.
- W1562983132 cites W2107511340 @default.
- W1562983132 cites W2110573224 @default.
- W1562983132 cites W2111427334 @default.
- W1562983132 cites W2112982595 @default.
- W1562983132 cites W2117392192 @default.
- W1562983132 cites W2119530061 @default.
- W1562983132 cites W2121698562 @default.
- W1562983132 cites W2123621585 @default.
- W1562983132 cites W2134245322 @default.
- W1562983132 cites W2134365313 @default.
- W1562983132 cites W2139997876 @default.
- W1562983132 cites W2141929638 @default.
- W1562983132 cites W2143146123 @default.
- W1562983132 cites W2149548143 @default.
- W1562983132 cites W2150606529 @default.
- W1562983132 cites W2152653529 @default.
- W1562983132 cites W2156451280 @default.
- W1562983132 cites W2165182802 @default.
- W1562983132 cites W2166642834 @default.
- W1562983132 cites W2169641775 @default.
- W1562983132 cites W2169835648 @default.
- W1562983132 cites W2318224232 @default.
- W1562983132 cites W2328701847 @default.
- W1562983132 doi "https://doi.org/10.3390/ijms160612513" @default.
- W1562983132 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4490458" @default.
- W1562983132 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/26047338" @default.
- W1562983132 hasPublicationYear "2015" @default.
- W1562983132 type Work @default.
- W1562983132 sameAs 1562983132 @default.
- W1562983132 citedByCount "35" @default.
- W1562983132 countsByYear W15629831322016 @default.
- W1562983132 countsByYear W15629831322017 @default.
- W1562983132 countsByYear W15629831322018 @default.
- W1562983132 countsByYear W15629831322019 @default.
- W1562983132 countsByYear W15629831322020 @default.
- W1562983132 countsByYear W15629831322021 @default.
- W1562983132 countsByYear W15629831322022 @default.
- W1562983132 countsByYear W15629831322023 @default.
- W1562983132 crossrefType "journal-article" @default.
- W1562983132 hasAuthorship W1562983132A5008166282 @default.
- W1562983132 hasAuthorship W1562983132A5011985227 @default.
- W1562983132 hasAuthorship W1562983132A5015538983 @default.
- W1562983132 hasAuthorship W1562983132A5020583473 @default.
- W1562983132 hasAuthorship W1562983132A5027148089 @default.
- W1562983132 hasAuthorship W1562983132A5037069151 @default.
- W1562983132 hasAuthorship W1562983132A5050739400 @default.
- W1562983132 hasAuthorship W1562983132A5068438174 @default.
- W1562983132 hasAuthorship W1562983132A5077466803 @default.
- W1562983132 hasBestOaLocation W15629831321 @default.
- W1562983132 hasConcept C104317684 @default.
- W1562983132 hasConcept C150194340 @default.
- W1562983132 hasConcept C2779148672 @default.
- W1562983132 hasConcept C48023723 @default.
- W1562983132 hasConcept C54355233 @default.
- W1562983132 hasConcept C86803240 @default.
- W1562983132 hasConceptScore W1562983132C104317684 @default.
- W1562983132 hasConceptScore W1562983132C150194340 @default.