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• W1563025174 abstract "Abstract The monomer of glutamine-dependent carbamyl phosphate synthetase can be reversibly dissociated to a heavy (mol wt ∼130,000) and a light (mol wt ∼42,000) subunit by treatment with 1 m potassium thiocyanate. The separated heavy subunit can catalyze carbamyl phosphate synthesis from ammonia but not from glutamine; it can also catalyze two of the partial reactions that are catalyzed by the native enzyme, i.e. bicarbonate-dependent ATP cleavage, and the synthesis of ATP from ADP and carbamyl phosphate. The only catalytic activity associated with the light subunit is hydrolysis of glutamine (or γ-glutamylhydroxamate). Recombination of the heavy and light subunits restores ability of the enzyme to utilize glutamine for carbamyl phosphate synthesis. The light and heavy subunits have been characterized by hydrodynamic, chemical, and enzymatic procedures. Evidence was obtained that the heavy subunit contains the sites for the allosteric activators (NH4+, IMP, ornithine) and the inhibitor UMP. The glutaminase activity exhibited by the light subunit exhibits an apparent Km value (130 to 180 mm) which is much higher than that found for the native enzyme or the reconstituted enzyme (1 mm). The effect of glutamine concentration on the glutaminase activity of the reconstituted enzyme and also of the native enzyme that has been stored for long periods was found to be the sum of two independent hyperbolic functions, indicating that in these enzyme preparations a portion of the molecules have a relatively low affinity for glutamine. Although the heavy subunit contains all of the structural properties required for carbamyl phosphate synthesis from ammonia (and the binding sites for allosteric effectors) and the light subunit functions to bind and cleave glutamine, the data indicate that there are substantial interactions between the subunits which in the native enzyme facilitate the utilization of glutamine for the synthesis of carbamyl phosphate." @default.
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• W1563025174 date "1974-01-01" @default.
• W1563025174 modified "2023-09-28" @default.
• W1563025174 title "Reversible Dissociation of the Monomer of Glutamine-dependent Carbamyl Phosphate Synthetase into Catalytically Active Heavy and Light Subunits" @default.
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• W1563025174 doi "https://doi.org/10.1016/s0021-9258(19)43057-3" @default.
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