FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1563360249> ?p ?o ?g. }

• W1563360249 endingPage "7843" @default.
• W1563360249 startingPage "7837" @default.
• W1563360249 abstract "Abstract Bisphosphoglycerate mutase (EC 5.4.2.4.) is a trifunctional enzyme which displays synthase, mutase, and phosphatase activities. The purification, characterization, and structural study of an abnormal form of the enzyme, isolated from a patient which we reported earlier (Rosa, R., Prehu, M. O., Beuzard, Y., and Rosa, J. (1978) J. Clin. Invest. 62, 907-915), is described. The abnormal enzyme, present at 50% of the level of the normal enzyme as estimated by immunological methods, showed elevated electrophoretic mobility and hybridized with erythrocyte phosphoglycerate mutase (EC 5.4.2.1.) in the same manner as the normal control. The mutant enzyme was unstable at 55 degrees C and could be protected against thermal instability by 0.5 mM glycerate 2,3-bisphoshate but not by either glycerate 3-phosphate or glycolate 2-phosphate. Two of the three functions of the mutant enzyme were distinct from those of the normal protein. The specific activity of the synthase was 0.57% of normal and that of the mutase 4.1%. By contrast, the specific phosphatase activity was not affected by the mutation. However, the phosphatase activity of the mutated protein was markedly less stimulated by glycolate-2-phosphate than that of the control. High performance liquid chromatography analysis of tryptic peptides derived from the mutant enzyme showed an abnormal profile with the absence of two peaks normally containing the T12 and T13 peptides and without the appearance of a supplementary peak. Amino acid sequence and mass spectrometric analysis demonstrated the substitution of Arg----Cys residue in position 89 producing an uncleaved T12-T13 present in the same peak as the T6. Considered together, our data suggest that Arg-89 is located at or near the active site of bisphosphoglycerate mutase and that this residue is probably involved in the binding of monophosphoglycerates." @default.
• W1563360249 created "2016-06-24" @default.
• W1563360249 creator A5028163940 @default.
• W1563360249 creator A5045044830 @default.
• W1563360249 creator A5052565614 @default.
• W1563360249 creator A5068824399 @default.
• W1563360249 creator A5073547885 @default.
• W1563360249 creator A5086432121 @default.
• W1563360249 date "1989-05-01" @default.
• W1563360249 modified "2023-10-13" @default.
• W1563360249 title "Isolation, characterization, and structure of a mutant 89 Arg → Cys bisphosphoglycerate mutase" @default.
• W1563360249 cites W1483221963 @default.
• W1563360249 cites W1495151785 @default.
• W1563360249 cites W1499750176 @default.
• W1563360249 cites W1544990785 @default.
• W1563360249 cites W1562626340 @default.
• W1563360249 cites W1587714218 @default.
• W1563360249 cites W1599053831 @default.
• W1563360249 cites W1675479680 @default.
• W1563360249 cites W1898371500 @default.
• W1563360249 cites W1915957948 @default.
• W1563360249 cites W1983675220 @default.
• W1563360249 cites W1991438254 @default.
• W1563360249 cites W1991559584 @default.
• W1563360249 cites W1994457355 @default.
• W1563360249 cites W2007802156 @default.
• W1563360249 cites W2010678341 @default.
• W1563360249 cites W2013744832 @default.
• W1563360249 cites W2017329508 @default.
• W1563360249 cites W2023269359 @default.
• W1563360249 cites W2030871360 @default.
• W1563360249 cites W2038474787 @default.
• W1563360249 cites W2049182812 @default.
• W1563360249 cites W2050123891 @default.
• W1563360249 cites W2066477562 @default.
• W1563360249 cites W2070737822 @default.
• W1563360249 cites W2071337331 @default.
• W1563360249 cites W2076122916 @default.
• W1563360249 cites W2083868361 @default.
• W1563360249 cites W2084268197 @default.
• W1563360249 cites W2100837269 @default.
• W1563360249 cites W2150784912 @default.
• W1563360249 cites W2153435210 @default.
• W1563360249 cites W1982382664 @default.
• W1563360249 cites W2052571295 @default.
• W1563360249 doi "https://doi.org/10.1016/s0021-9258(18)83118-0" @default.
• W1563360249 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/2542247" @default.
• W1563360249 hasPublicationYear "1989" @default.
• W1563360249 type Work @default.
• W1563360249 sameAs 1563360249 @default.
• W1563360249 citedByCount "25" @default.
• W1563360249 countsByYear W15633602492013 @default.
• W1563360249 countsByYear W15633602492014 @default.
• W1563360249 countsByYear W15633602492017 @default.
• W1563360249 countsByYear W15633602492018 @default.
• W1563360249 countsByYear W15633602492020 @default.
• W1563360249 crossrefType "journal-article" @default.
• W1563360249 hasAuthorship W1563360249A5028163940 @default.
• W1563360249 hasAuthorship W1563360249A5045044830 @default.
• W1563360249 hasAuthorship W1563360249A5052565614 @default.
• W1563360249 hasAuthorship W1563360249A5068824399 @default.
• W1563360249 hasAuthorship W1563360249A5073547885 @default.
• W1563360249 hasAuthorship W1563360249A5086432121 @default.
• W1563360249 hasBestOaLocation W15633602491 @default.
• W1563360249 hasConcept C104317684 @default.
• W1563360249 hasConcept C143065580 @default.
• W1563360249 hasConcept C171250308 @default.
• W1563360249 hasConcept C181199279 @default.
• W1563360249 hasConcept C185592680 @default.
• W1563360249 hasConcept C192562407 @default.
• W1563360249 hasConcept C20251656 @default.
• W1563360249 hasConcept C2775941552 @default.
• W1563360249 hasConcept C2777552656 @default.
• W1563360249 hasConcept C2780841128 @default.
• W1563360249 hasConcept C55493867 @default.
• W1563360249 hasConcept C86803240 @default.
• W1563360249 hasConcept C89423630 @default.
• W1563360249 hasConcept C91754966 @default.
• W1563360249 hasConceptScore W1563360249C104317684 @default.
• W1563360249 hasConceptScore W1563360249C143065580 @default.
• W1563360249 hasConceptScore W1563360249C171250308 @default.
• W1563360249 hasConceptScore W1563360249C181199279 @default.
• W1563360249 hasConceptScore W1563360249C185592680 @default.
• W1563360249 hasConceptScore W1563360249C192562407 @default.
• W1563360249 hasConceptScore W1563360249C20251656 @default.
• W1563360249 hasConceptScore W1563360249C2775941552 @default.
• W1563360249 hasConceptScore W1563360249C2777552656 @default.
• W1563360249 hasConceptScore W1563360249C2780841128 @default.
• W1563360249 hasConceptScore W1563360249C55493867 @default.
• W1563360249 hasConceptScore W1563360249C86803240 @default.
• W1563360249 hasConceptScore W1563360249C89423630 @default.
• W1563360249 hasConceptScore W1563360249C91754966 @default.
• W1563360249 hasIssue "14" @default.
• W1563360249 hasLocation W15633602491 @default.
• W1563360249 hasOpenAccess W1563360249 @default.
• W1563360249 hasPrimaryLocation W15633602491 @default.
• W1563360249 hasRelatedWork W1556023848 @default.
• W1563360249 hasRelatedWork W1976078405 @default.

• next