Matches in SemOpenAlex for { <https://semopenalex.org/work/W1564262015> ?p ?o ?g. }
Showing items 1 to 100 of
100
with 100 items per page.
- W1564262015 abstract "The aim of the present work was to develop a sensitive and specific real-time PCR diagnostic system for the rapid detection of bovine adenovirus in cattle. A TaqMan real-time PCR assay capable of simultaneous detection of nine out of ten species of bovine adenovirus belonging to the genera Atadenovirus and Mastadenovirus was developed. The oligonucleotide primers and probe were selected from conserved sequences flanking the genome region coding for the Hexon protein. DNA from BAdV species 1 to 10 was isolated by standard phenol-chloroform extraction. Standard curve using a ten-fold dilution series of BAdV-8 positive DNA template was generated. The sensitivity of assay was determined using BAV-8 cloned PCR product and the detection limit was five copies of viral genome equivalents. The efficiency of PCR assay was 97%. Only BAdV-2 serotype was not possible to detect and BAdV-7 has shown low amplification rates and fluorescence, but anyway detected, due to four mismatches in the probe region. In conclusion, the TaqMan real-time PCR assay presented here is expected to be a good alternative tool to the traditional diagnostic methods like virus isolation, ELISA or conventional PCR in case of sporadic disease outbreak in which rapid, sensitive and specific diagnosis is required and when appropriate measures should be taken." @default.
- W1564262015 created "2016-06-24" @default.
- W1564262015 creator A5018771892 @default.
- W1564262015 date "2011-10-25" @default.
- W1564262015 modified "2023-09-27" @default.
- W1564262015 title "Detection of adenoviruses in cattle" @default.
- W1564262015 cites W120472992 @default.
- W1564262015 cites W1600895471 @default.
- W1564262015 cites W1608933347 @default.
- W1564262015 cites W1986571323 @default.
- W1564262015 cites W1996235128 @default.
- W1564262015 cites W2010585539 @default.
- W1564262015 cites W2020430828 @default.
- W1564262015 cites W2020630733 @default.
- W1564262015 cites W2028240922 @default.
- W1564262015 cites W2030155366 @default.
- W1564262015 cites W2035946392 @default.
- W1564262015 cites W2042073917 @default.
- W1564262015 cites W2074471872 @default.
- W1564262015 cites W2074594679 @default.
- W1564262015 cites W2076032948 @default.
- W1564262015 cites W2087510109 @default.
- W1564262015 cites W2101240852 @default.
- W1564262015 cites W2120544209 @default.
- W1564262015 cites W2125881398 @default.
- W1564262015 cites W2127309703 @default.
- W1564262015 cites W2169310464 @default.
- W1564262015 cites W2326350436 @default.
- W1564262015 cites W2339207115 @default.
- W1564262015 cites W2396425856 @default.
- W1564262015 cites W2417632234 @default.
- W1564262015 cites W2425329571 @default.
- W1564262015 cites W266942110 @default.
- W1564262015 cites W2974114797 @default.
- W1564262015 cites W587625591 @default.
- W1564262015 cites W2800729038 @default.
- W1564262015 cites W2988045179 @default.
- W1564262015 hasPublicationYear "2011" @default.
- W1564262015 type Work @default.
- W1564262015 sameAs 1564262015 @default.
- W1564262015 citedByCount "0" @default.
- W1564262015 crossrefType "journal-article" @default.
- W1564262015 hasAuthorship W1564262015A5018771892 @default.
- W1564262015 hasConcept C104317684 @default.
- W1564262015 hasConcept C119128265 @default.
- W1564262015 hasConcept C129312508 @default.
- W1564262015 hasConcept C141231307 @default.
- W1564262015 hasConcept C143660080 @default.
- W1564262015 hasConcept C153911025 @default.
- W1564262015 hasConcept C159047783 @default.
- W1564262015 hasConcept C185592680 @default.
- W1564262015 hasConcept C43617362 @default.
- W1564262015 hasConcept C48023723 @default.
- W1564262015 hasConcept C49105822 @default.
- W1564262015 hasConcept C54355233 @default.
- W1564262015 hasConcept C552990157 @default.
- W1564262015 hasConcept C78063203 @default.
- W1564262015 hasConcept C86803240 @default.
- W1564262015 hasConceptScore W1564262015C104317684 @default.
- W1564262015 hasConceptScore W1564262015C119128265 @default.
- W1564262015 hasConceptScore W1564262015C129312508 @default.
- W1564262015 hasConceptScore W1564262015C141231307 @default.
- W1564262015 hasConceptScore W1564262015C143660080 @default.
- W1564262015 hasConceptScore W1564262015C153911025 @default.
- W1564262015 hasConceptScore W1564262015C159047783 @default.
- W1564262015 hasConceptScore W1564262015C185592680 @default.
- W1564262015 hasConceptScore W1564262015C43617362 @default.
- W1564262015 hasConceptScore W1564262015C48023723 @default.
- W1564262015 hasConceptScore W1564262015C49105822 @default.
- W1564262015 hasConceptScore W1564262015C54355233 @default.
- W1564262015 hasConceptScore W1564262015C552990157 @default.
- W1564262015 hasConceptScore W1564262015C78063203 @default.
- W1564262015 hasConceptScore W1564262015C86803240 @default.
- W1564262015 hasLocation W15642620151 @default.
- W1564262015 hasOpenAccess W1564262015 @default.
- W1564262015 hasPrimaryLocation W15642620151 @default.
- W1564262015 hasRelatedWork W1017736069 @default.
- W1564262015 hasRelatedWork W1191261996 @default.
- W1564262015 hasRelatedWork W1965948166 @default.
- W1564262015 hasRelatedWork W199907031 @default.
- W1564262015 hasRelatedWork W2000837270 @default.
- W1564262015 hasRelatedWork W2048940051 @default.
- W1564262015 hasRelatedWork W2140455798 @default.
- W1564262015 hasRelatedWork W2141166342 @default.
- W1564262015 hasRelatedWork W2234447393 @default.
- W1564262015 hasRelatedWork W2350255361 @default.
- W1564262015 hasRelatedWork W2352892736 @default.
- W1564262015 hasRelatedWork W2357502694 @default.
- W1564262015 hasRelatedWork W2377040750 @default.
- W1564262015 hasRelatedWork W2383732964 @default.
- W1564262015 hasRelatedWork W2392577013 @default.
- W1564262015 hasRelatedWork W2395926632 @default.
- W1564262015 hasRelatedWork W2415719425 @default.
- W1564262015 hasRelatedWork W2989730137 @default.
- W1564262015 hasRelatedWork W3028930990 @default.
- W1564262015 hasRelatedWork W833287666 @default.
- W1564262015 isParatext "false" @default.
- W1564262015 isRetracted "false" @default.
- W1564262015 magId "1564262015" @default.
- W1564262015 workType "article" @default.