Matches in SemOpenAlex for { <https://semopenalex.org/work/W156428054> ?p ?o ?g. }
- W156428054 endingPage "541" @default.
- W156428054 startingPage "505" @default.
- W156428054 abstract "Recent developments in DNA vaccine research provide a new momentum for this rather young and potentially disruptive technology. Gene-based vaccines are capable of eliciting protective immunity in humans to persistent intracellular pathogens, such as HIV, malaria, and tuberculosis, for which the conventional vaccine technologies have failed so far. The recent identification and characterization of genes coding for tumor antigens has stimulated the development of DNA-based antigen-specific cancer vaccines. Although most academic researchers consider the production of reasonable amounts of plasmid DNA (pDNA) for immunological studies relatively easy to solve, problems often arise during this first phase of production. In this chapter we review the current state of the art of pDNA production at small (shake flasks) and mid-scales (lab-scale bioreactor fermentations) and address new trends in vector design and strain engineering. We will guide the reader through the different stages of process design starting from choosing the most appropriate plasmid backbone, choosing the right Escherichia coli (E. coli) strain for production, and cultivation media and scale-up issues. In addition, we will address some points concerning the safety and potency of the produced plasmids, with special focus on producing antibiotic resistance-free plasmids. The main goal of this chapter is to make immunologists aware of the fact that production of the pDNA vaccine has to be performed with as much as attention and care as the rest of their research." @default.
- W156428054 created "2016-06-24" @default.
- W156428054 creator A5016123662 @default.
- W156428054 creator A5016656069 @default.
- W156428054 date "2014-01-01" @default.
- W156428054 modified "2023-10-18" @default.
- W156428054 title "Advances in Host and Vector Development for the Production of Plasmid DNA Vaccines" @default.
- W156428054 cites W1427184295 @default.
- W156428054 cites W1485108234 @default.
- W156428054 cites W1507226542 @default.
- W156428054 cites W1514335381 @default.
- W156428054 cites W1514540900 @default.
- W156428054 cites W156963000 @default.
- W156428054 cites W1656835564 @default.
- W156428054 cites W167353705 @default.
- W156428054 cites W170489579 @default.
- W156428054 cites W1724214647 @default.
- W156428054 cites W1807212217 @default.
- W156428054 cites W183414650 @default.
- W156428054 cites W1970972784 @default.
- W156428054 cites W1971441722 @default.
- W156428054 cites W1972603233 @default.
- W156428054 cites W197289586 @default.
- W156428054 cites W1972915118 @default.
- W156428054 cites W1973125157 @default.
- W156428054 cites W1973402866 @default.
- W156428054 cites W1973503110 @default.
- W156428054 cites W1974129985 @default.
- W156428054 cites W1975049168 @default.
- W156428054 cites W1976189088 @default.
- W156428054 cites W1976270442 @default.
- W156428054 cites W1977358869 @default.
- W156428054 cites W1977901209 @default.
- W156428054 cites W1979250710 @default.
- W156428054 cites W1981906993 @default.
- W156428054 cites W1982274303 @default.
- W156428054 cites W1983511787 @default.
- W156428054 cites W1983546677 @default.
- W156428054 cites W1985505095 @default.
- W156428054 cites W1987246047 @default.
- W156428054 cites W1988414113 @default.
- W156428054 cites W1988723349 @default.
- W156428054 cites W1988746752 @default.
- W156428054 cites W1989007217 @default.
- W156428054 cites W1989071313 @default.
- W156428054 cites W1990046217 @default.
- W156428054 cites W1993613957 @default.
- W156428054 cites W1993660586 @default.
- W156428054 cites W1994294376 @default.
- W156428054 cites W1994503800 @default.
- W156428054 cites W1995924881 @default.
- W156428054 cites W1996327915 @default.
- W156428054 cites W1997366820 @default.
- W156428054 cites W1999256200 @default.
- W156428054 cites W1999275494 @default.
- W156428054 cites W2000122942 @default.
- W156428054 cites W2000260352 @default.
- W156428054 cites W2002759609 @default.
- W156428054 cites W2005543617 @default.
- W156428054 cites W2006846934 @default.
- W156428054 cites W2006906132 @default.
- W156428054 cites W2007146866 @default.
- W156428054 cites W2009560463 @default.
- W156428054 cites W2010748293 @default.
- W156428054 cites W2011979066 @default.
- W156428054 cites W2014395129 @default.
- W156428054 cites W2017224563 @default.
- W156428054 cites W2019410656 @default.
- W156428054 cites W2020966401 @default.
- W156428054 cites W2021693767 @default.
- W156428054 cites W2021869112 @default.
- W156428054 cites W2024010585 @default.
- W156428054 cites W2025735204 @default.
- W156428054 cites W2027084198 @default.
- W156428054 cites W2027151731 @default.
- W156428054 cites W2027717772 @default.
- W156428054 cites W2029822304 @default.
- W156428054 cites W2031485466 @default.
- W156428054 cites W2032555936 @default.
- W156428054 cites W2032656068 @default.
- W156428054 cites W2032776824 @default.
- W156428054 cites W2035078651 @default.
- W156428054 cites W2035435023 @default.
- W156428054 cites W2036006268 @default.
- W156428054 cites W2036110284 @default.
- W156428054 cites W2037338661 @default.
- W156428054 cites W2037587513 @default.
- W156428054 cites W2037999785 @default.
- W156428054 cites W2038554879 @default.
- W156428054 cites W2039971319 @default.
- W156428054 cites W2041778510 @default.
- W156428054 cites W2042262705 @default.
- W156428054 cites W2043076197 @default.
- W156428054 cites W2043247452 @default.
- W156428054 cites W2044318184 @default.
- W156428054 cites W2044686496 @default.
- W156428054 cites W20456437 @default.
- W156428054 cites W2045937044 @default.