SemOpenAlex |

SemOpenAlex

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1564511283> ?p ?o ?g. }

Showing items 1 to 100 of 100 with 100 items per page.

W1564511283 endingPage "109" @default.
W1564511283 startingPage "104" @default.
W1564511283 abstract "To assess a pedicled gracilis muscle flap pre-laminated with autologous, in vitro-expanded urothelial cells to reconstruct an entire supratrigonal bladder-wall defect in rats.A gracilis muscle flap was harvested from 36 male Wistar rats, transposed into the abdomen and wrapped around a silicon-block space holder. Urothelial cells were harvested and expanded ex vivo. Cells were then suspended in fibrin glue and seeded into the gracilis muscle pocket. One week later this pre-laminated flap was transposed into a surgically created supratrigonal bladder-wall defect. All animals underwent such a pre-laminated gracilis flap bladder reconstruction and were categorized into three experimental groups. All surviving animals with urothelial-culture pre-laminated gracilis flap bladder reconstruction were killed 12 weeks (group 1) later. Control rats had gracilis flaps with no cell seeding and treated only with fibrin glue (group 2) or a standard culture medium (group 3) before reconstruction.Specimens stained with haematoxylin and eosin, and a specific immunohistochemical staining (AE1 and AE3-anti-cytokeratin monoclonal antibody stain) showed a continuous, multilayered functioning urothelial lining along the transposed pre-laminated gracilis flap in group 1. All animals in group 1 with an intact urothelial lining on the gracilis muscle survived, in contrast to most animals in groups 2 and 3, where eight and all 12 animals died, respectively. The surviving four animals in group 2 had no detectable urothelial lining. CONCLUSION Successful urinary reconstruction requires a contractile neo-reservoir resistant to resorption over time and a stable, protective urothelial lining. A gracilis muscle flap can be seeded with autologous cultured urothelial cells suspended in fibrin glue. This pre-laminated flap can be safely transposed on its pedicle and be successfully integrated into the remaining bladder wall, with a urothelial lining and the potential to contract. Further studies in larger animals, with a urodynamic assessment, are warranted to determine if this type of bladder-wall replacement technique is suitable for urinary reconstruction in humans." @default.
W1564511283 created "2016-06-24" @default.
W1564511283 creator A5013058168 @default.
W1564511283 creator A5057290567 @default.
W1564511283 creator A5059168977 @default.
W1564511283 creator A5063459190 @default.
W1564511283 creator A5086080945 @default.
W1564511283 date "2001-07-01" @default.
W1564511283 modified "2023-09-23" @default.
W1564511283 title "Gracilis muscle flap with a tissue-engineered lining for experimental bladder wall reconstruction" @default.
W1564511283 cites W1223761686 @default.
W1564511283 cites W1972668076 @default.
W1564511283 cites W1991445463 @default.
W1564511283 cites W1992113353 @default.
W1564511283 cites W2006742647 @default.
W1564511283 cites W2013861830 @default.
W1564511283 cites W2023969462 @default.
W1564511283 cites W2035702825 @default.
W1564511283 cites W2071826231 @default.
W1564511283 cites W2084002400 @default.
W1564511283 cites W2094873483 @default.
W1564511283 cites W2118886001 @default.
W1564511283 cites W2142259238 @default.
W1564511283 cites W2248442723 @default.
W1564511283 cites W2286715646 @default.
W1564511283 cites W2397596469 @default.
W1564511283 cites W2409609971 @default.
W1564511283 cites W2416217797 @default.
W1564511283 cites W2426310001 @default.
W1564511283 cites W2429485198 @default.
W1564511283 doi "https://doi.org/10.1046/j.1464-410x.2001.02237.x" @default.
W1564511283 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/11446857" @default.
W1564511283 hasPublicationYear "2001" @default.
W1564511283 type Work @default.
W1564511283 sameAs 1564511283 @default.
W1564511283 citedByCount "6" @default.
W1564511283 crossrefType "journal-article" @default.
W1564511283 hasAuthorship W1564511283A5013058168 @default.
W1564511283 hasAuthorship W1564511283A5057290567 @default.
W1564511283 hasAuthorship W1564511283A5059168977 @default.
W1564511283 hasAuthorship W1564511283A5063459190 @default.
W1564511283 hasAuthorship W1564511283A5086080945 @default.
W1564511283 hasConcept C105702510 @default.
W1564511283 hasConcept C141071460 @default.
W1564511283 hasConcept C142724271 @default.
W1564511283 hasConcept C204232928 @default.
W1564511283 hasConcept C2776919888 @default.
W1564511283 hasConcept C2777540882 @default.
W1564511283 hasConcept C2779012181 @default.
W1564511283 hasConcept C2779762690 @default.
W1564511283 hasConcept C2780371428 @default.
W1564511283 hasConcept C2781472170 @default.
W1564511283 hasConcept C2910041100 @default.
W1564511283 hasConcept C71924100 @default.
W1564511283 hasConcept C77411442 @default.
W1564511283 hasConceptScore W1564511283C105702510 @default.
W1564511283 hasConceptScore W1564511283C141071460 @default.
W1564511283 hasConceptScore W1564511283C142724271 @default.
W1564511283 hasConceptScore W1564511283C204232928 @default.
W1564511283 hasConceptScore W1564511283C2776919888 @default.
W1564511283 hasConceptScore W1564511283C2777540882 @default.
W1564511283 hasConceptScore W1564511283C2779012181 @default.
W1564511283 hasConceptScore W1564511283C2779762690 @default.
W1564511283 hasConceptScore W1564511283C2780371428 @default.
W1564511283 hasConceptScore W1564511283C2781472170 @default.
W1564511283 hasConceptScore W1564511283C2910041100 @default.
W1564511283 hasConceptScore W1564511283C71924100 @default.
W1564511283 hasConceptScore W1564511283C77411442 @default.
W1564511283 hasIssue "1" @default.
W1564511283 hasLocation W15645112831 @default.
W1564511283 hasLocation W15645112832 @default.
W1564511283 hasOpenAccess W1564511283 @default.
W1564511283 hasPrimaryLocation W15645112831 @default.
W1564511283 hasRelatedWork W1896732586 @default.
W1564511283 hasRelatedWork W1936146733 @default.
W1564511283 hasRelatedWork W2007801361 @default.
W1564511283 hasRelatedWork W2023281560 @default.
W1564511283 hasRelatedWork W2024244262 @default.
W1564511283 hasRelatedWork W2032032405 @default.
W1564511283 hasRelatedWork W2043307943 @default.
W1564511283 hasRelatedWork W2046147687 @default.
W1564511283 hasRelatedWork W2050296002 @default.
W1564511283 hasRelatedWork W2050617554 @default.
W1564511283 hasRelatedWork W2056097048 @default.
W1564511283 hasRelatedWork W2064658415 @default.
W1564511283 hasRelatedWork W2071826231 @default.
W1564511283 hasRelatedWork W2087027348 @default.
W1564511283 hasRelatedWork W2088051435 @default.
W1564511283 hasRelatedWork W2356783827 @default.
W1564511283 hasRelatedWork W2357404586 @default.
W1564511283 hasRelatedWork W2365960075 @default.
W1564511283 hasRelatedWork W2367961481 @default.
W1564511283 hasRelatedWork W2378377610 @default.
W1564511283 hasVolume "88" @default.
W1564511283 isParatext "false" @default.
W1564511283 isRetracted "false" @default.
W1564511283 magId "1564511283" @default.
W1564511283 workType "article" @default.