Matches in SemOpenAlex for { <https://semopenalex.org/work/W1564674591> ?p ?o ?g. }
- W1564674591 abstract "Cell fractionation techniques include some of the most important and widely used analytical tools in cell and molecular biology, and are essential for the development of cell-free assays that reconstitute complicated cellular processes. In addition to simple gradient systems, this unit discusses the immuno-purification of organelles, in particular endosomes. As antigens, purification can be achieved using endogenous or ectopically expressed proteins, provided that appropriate antibodies are available. Alternatively, tagged proteins can be used, when combined with anti-tag antibodies. Now that sequencing of the genomes of several organisms has been completed, biochemical strategies, and in particular fractionation and in vitro transport assays, are more necessary than ever to study the numerous protein and protein complexes that are being discovered." @default.
- W1564674591 created "2016-06-24" @default.
- W1564674591 creator A5033432789 @default.
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- W1564674591 date "2003-05-01" @default.
- W1564674591 modified "2023-10-12" @default.
- W1564674591 title "Subcellular Fractionation of Tissue Culture Cells" @default.
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- W1564674591 doi "https://doi.org/10.1002/0471140864.ps0403s32" @default.
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