Matches in SemOpenAlex for { <https://semopenalex.org/work/W1565431211> ?p ?o ?g. }
- W1565431211 abstract "SIRT1, the founding member of the mammalian family of seven NAD(+)-dependent sirtuins, is composed of 747 amino acids forming a catalytic domain and extended N- and C-terminal regions. We report the design and characterization of an engineered human SIRT1 construct (mini-hSIRT1) containing the minimal structural elements required for lysine deacetylation and catalytic activation by small molecule sirtuin-activating compounds (STACs). Using this construct, we solved the crystal structure of a mini-hSIRT1-STAC complex, which revealed the STAC-binding site within the N-terminal domain of hSIRT1. Together with hydrogen-deuterium exchange mass spectrometry (HDX-MS) and site-directed mutagenesis using full-length hSIRT1, these data establish a specific STAC-binding site and identify key intermolecular interactions with hSIRT1. The determination of the interface governing the binding of STACs with human SIRT1 facilitates greater understanding of STAC activation of this enzyme, which holds significant promise as a therapeutic target for multiple human diseases." @default.
- W1565431211 created "2016-06-24" @default.
- W1565431211 creator A5003694271 @default.
- W1565431211 creator A5005646933 @default.
- W1565431211 creator A5007236317 @default.
- W1565431211 creator A5008455101 @default.
- W1565431211 creator A5009317615 @default.
- W1565431211 creator A5009819299 @default.
- W1565431211 creator A5011631358 @default.
- W1565431211 creator A5011819247 @default.
- W1565431211 creator A5014046875 @default.
- W1565431211 creator A5016503620 @default.
- W1565431211 creator A5018852320 @default.
- W1565431211 creator A5019519248 @default.
- W1565431211 creator A5021486255 @default.
- W1565431211 creator A5022079431 @default.
- W1565431211 creator A5032612285 @default.
- W1565431211 creator A5036484672 @default.
- W1565431211 creator A5036647389 @default.
- W1565431211 creator A5036837044 @default.
- W1565431211 creator A5037803523 @default.
- W1565431211 creator A5041988653 @default.
- W1565431211 creator A5043226647 @default.
- W1565431211 creator A5045322500 @default.
- W1565431211 creator A5045580713 @default.
- W1565431211 creator A5046523772 @default.
- W1565431211 creator A5048474774 @default.
- W1565431211 creator A5050626806 @default.
- W1565431211 creator A5053987355 @default.
- W1565431211 creator A5065473351 @default.
- W1565431211 creator A5065591698 @default.
- W1565431211 creator A5074314664 @default.
- W1565431211 creator A5075806152 @default.
- W1565431211 creator A5077605906 @default.
- W1565431211 creator A5085167417 @default.
- W1565431211 date "2015-07-02" @default.
- W1565431211 modified "2023-10-01" @default.
- W1565431211 title "Crystallographic structure of a small molecule SIRT1 activator-enzyme complex" @default.
- W1565431211 cites W1985970694 @default.
- W1565431211 cites W1986511022 @default.
- W1565431211 cites W1990931780 @default.
- W1565431211 cites W1992823976 @default.
- W1565431211 cites W1996395636 @default.
- W1565431211 cites W2003623751 @default.
- W1565431211 cites W2006922696 @default.
- W1565431211 cites W2030955780 @default.
- W1565431211 cites W2032434211 @default.
- W1565431211 cites W2041959419 @default.
- W1565431211 cites W2043901123 @default.
- W1565431211 cites W2051960831 @default.
- W1565431211 cites W2053143505 @default.
- W1565431211 cites W2063612483 @default.
- W1565431211 cites W2080192652 @default.
- W1565431211 cites W2083378095 @default.
- W1565431211 cites W2087019732 @default.
- W1565431211 cites W2088381487 @default.
- W1565431211 cites W2088958016 @default.
- W1565431211 cites W2095997160 @default.
- W1565431211 cites W2097042870 @default.
- W1565431211 cites W2100649471 @default.
- W1565431211 cites W2100978260 @default.
- W1565431211 cites W2102643362 @default.
- W1565431211 cites W2109184569 @default.
- W1565431211 cites W2116350182 @default.
- W1565431211 cites W2116965394 @default.
- W1565431211 cites W2118964113 @default.
- W1565431211 cites W2133727193 @default.
- W1565431211 cites W2135711139 @default.
- W1565431211 cites W2142402729 @default.
- W1565431211 cites W2144081223 @default.
- W1565431211 cites W2157860216 @default.
- W1565431211 cites W2163341755 @default.
- W1565431211 cites W2166529463 @default.
- W1565431211 cites W88247729 @default.
- W1565431211 doi "https://doi.org/10.1038/ncomms8645" @default.
- W1565431211 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4506539" @default.
- W1565431211 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/26134520" @default.
- W1565431211 hasPublicationYear "2015" @default.
- W1565431211 type Work @default.
- W1565431211 sameAs 1565431211 @default.
- W1565431211 citedByCount "137" @default.
- W1565431211 countsByYear W15654312112015 @default.
- W1565431211 countsByYear W15654312112016 @default.
- W1565431211 countsByYear W15654312112017 @default.
- W1565431211 countsByYear W15654312112018 @default.
- W1565431211 countsByYear W15654312112019 @default.
- W1565431211 countsByYear W15654312112020 @default.
- W1565431211 countsByYear W15654312112021 @default.
- W1565431211 countsByYear W15654312112022 @default.
- W1565431211 countsByYear W15654312112023 @default.
- W1565431211 crossrefType "journal-article" @default.
- W1565431211 hasAuthorship W1565431211A5003694271 @default.
- W1565431211 hasAuthorship W1565431211A5005646933 @default.
- W1565431211 hasAuthorship W1565431211A5007236317 @default.
- W1565431211 hasAuthorship W1565431211A5008455101 @default.
- W1565431211 hasAuthorship W1565431211A5009317615 @default.
- W1565431211 hasAuthorship W1565431211A5009819299 @default.
- W1565431211 hasAuthorship W1565431211A5011631358 @default.
- W1565431211 hasAuthorship W1565431211A5011819247 @default.
- W1565431211 hasAuthorship W1565431211A5014046875 @default.