Matches in SemOpenAlex for { <https://semopenalex.org/work/W1565494682> ?p ?o ?g. }
- W1565494682 endingPage "9515" @default.
- W1565494682 startingPage "9512" @default.
- W1565494682 abstract "The lip gene of Escherichia coli has been cloned and sequenced. Subcloning of a 3-kilobase EcoRI/EcoRV restriction fragment from Clark-Carbon plasmid pLC15-5 into pUC18 gives a plasmid that complements two lipoate auxotrophs, W1485-lip2 and JRG33-lip9, and which expresses a protein of approximately 36,000 Da. Sequencing suggests that lip codes for a protein of 281 amino acids (31,350 Da), showing sequence similarity to biotin synthase. It is thus likely that lip encodes a sulfur insertion enzyme analogous to biotin synthase and that the sulfur insertion chemistries of the two systems are related. Unidirectional nested deletion experiments show that both lipoate auxotrophs are complemented by the same 500-base pair region at the 3' terminus of the lip gene, indicating that the mutations affecting lipoate biosynthesis are located in this region of the protein. A small open reading frame located immediately downstream of the lip gene codes for a small protein of unknown function." @default.
- W1565494682 created "2016-06-24" @default.
- W1565494682 creator A5013413764 @default.
- W1565494682 creator A5016595929 @default.
- W1565494682 creator A5071278593 @default.
- W1565494682 creator A5082839466 @default.
- W1565494682 date "1992-05-01" @default.
- W1565494682 modified "2023-09-27" @default.
- W1565494682 title "The biosynthesis of lipoic acid. Cloning of lip, a lipoate biosynthetic locus of Escherichia coli." @default.
- W1565494682 cites W1517573369 @default.
- W1565494682 cites W1525121830 @default.
- W1565494682 cites W1539733232 @default.
- W1565494682 cites W1546636541 @default.
- W1565494682 cites W1557719847 @default.
- W1565494682 cites W1566689904 @default.
- W1565494682 cites W1588077437 @default.
- W1565494682 cites W1609528353 @default.
- W1565494682 cites W1971095040 @default.
- W1565494682 cites W1974495245 @default.
- W1565494682 cites W1977119229 @default.
- W1565494682 cites W1977767379 @default.
- W1565494682 cites W1979801633 @default.
- W1565494682 cites W1983438549 @default.
- W1565494682 cites W1993243592 @default.
- W1565494682 cites W2005909790 @default.
- W1565494682 cites W2014495927 @default.
- W1565494682 cites W2023270541 @default.
- W1565494682 cites W2034184460 @default.
- W1565494682 cites W2037588355 @default.
- W1565494682 cites W2046204080 @default.
- W1565494682 cites W2050251634 @default.
- W1565494682 cites W2070237277 @default.
- W1565494682 cites W2070293940 @default.
- W1565494682 cites W2091216861 @default.
- W1565494682 cites W2093923504 @default.
- W1565494682 cites W2096367521 @default.
- W1565494682 cites W2104941760 @default.
- W1565494682 cites W2114452310 @default.
- W1565494682 cites W2121989806 @default.
- W1565494682 cites W2122967431 @default.
- W1565494682 cites W2150817394 @default.
- W1565494682 cites W2164356347 @default.
- W1565494682 cites W2165151174 @default.
- W1565494682 cites W4229875530 @default.
- W1565494682 cites W4293247451 @default.
- W1565494682 cites W7532581 @default.
- W1565494682 doi "https://doi.org/10.1016/s0021-9258(19)50120-x" @default.
- W1565494682 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/1577793" @default.
- W1565494682 hasPublicationYear "1992" @default.
- W1565494682 type Work @default.
- W1565494682 sameAs 1565494682 @default.
- W1565494682 citedByCount "52" @default.
- W1565494682 countsByYear W15654946822013 @default.
- W1565494682 countsByYear W15654946822014 @default.
- W1565494682 countsByYear W15654946822015 @default.
- W1565494682 countsByYear W15654946822016 @default.
- W1565494682 countsByYear W15654946822020 @default.
- W1565494682 crossrefType "journal-article" @default.
- W1565494682 hasAuthorship W1565494682A5013413764 @default.
- W1565494682 hasAuthorship W1565494682A5016595929 @default.
- W1565494682 hasAuthorship W1565494682A5071278593 @default.
- W1565494682 hasAuthorship W1565494682A5082839466 @default.
- W1565494682 hasBestOaLocation W15654946821 @default.
- W1565494682 hasConcept C104317684 @default.
- W1565494682 hasConcept C121050878 @default.
- W1565494682 hasConcept C185592680 @default.
- W1565494682 hasConcept C199360897 @default.
- W1565494682 hasConcept C2778004101 @default.
- W1565494682 hasConcept C2779491571 @default.
- W1565494682 hasConcept C41008148 @default.
- W1565494682 hasConcept C547475151 @default.
- W1565494682 hasConcept C553450214 @default.
- W1565494682 hasConcept C55493867 @default.
- W1565494682 hasConcept C84597430 @default.
- W1565494682 hasConcept C86803240 @default.
- W1565494682 hasConceptScore W1565494682C104317684 @default.
- W1565494682 hasConceptScore W1565494682C121050878 @default.
- W1565494682 hasConceptScore W1565494682C185592680 @default.
- W1565494682 hasConceptScore W1565494682C199360897 @default.
- W1565494682 hasConceptScore W1565494682C2778004101 @default.
- W1565494682 hasConceptScore W1565494682C2779491571 @default.
- W1565494682 hasConceptScore W1565494682C41008148 @default.
- W1565494682 hasConceptScore W1565494682C547475151 @default.
- W1565494682 hasConceptScore W1565494682C553450214 @default.
- W1565494682 hasConceptScore W1565494682C55493867 @default.
- W1565494682 hasConceptScore W1565494682C84597430 @default.
- W1565494682 hasConceptScore W1565494682C86803240 @default.
- W1565494682 hasIssue "14" @default.
- W1565494682 hasLocation W15654946821 @default.
- W1565494682 hasOpenAccess W1565494682 @default.
- W1565494682 hasPrimaryLocation W15654946821 @default.
- W1565494682 hasRelatedWork W1486372651 @default.
- W1565494682 hasRelatedWork W1508691356 @default.
- W1565494682 hasRelatedWork W1557635596 @default.
- W1565494682 hasRelatedWork W1751206423 @default.
- W1565494682 hasRelatedWork W1995986374 @default.
- W1565494682 hasRelatedWork W2017285598 @default.
- W1565494682 hasRelatedWork W2050917233 @default.