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- W1565641572 abstract "Abstract Peptides are important biomolecules that play a variety of functional roles. Knowledge of the specific sequence of amino acids in peptides is essential to understand their biological functions, to identify them in target tissues, to map changes in their metabolic profile, to assess the purity of newly synthesized peptides and to characterize their precursor proteins. Currently, mass spectrometry has assumed a pivotal role in determining the sequence of peptides. The procedure involves conversion of peptide molecules into gas‐phase ions, fragmentation of these species into the sequence‐specific ions, and deciphering the mass spectral data into the sequence of the target peptide. This procedure, known as “ de novo sequencing”, requires understanding of the peptide fragmentation rules and guidelines for interpretation of a mass spectrum. These aspects are discussed in detail in this chapter." @default.
- W1565641572 created "2016-06-24" @default.
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- W1565641572 date "2009-03-13" @default.
- W1565641572 modified "2023-10-10" @default.
- W1565641572 title "De Novo Sequencing of Peptides Using Mass Spectrometry" @default.
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- W1565641572 doi "https://doi.org/10.1002/9780470048672.wecb720" @default.
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