FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1565701814> ?p ?o ?g. }

• W1565701814 endingPage "9" @default.
• W1565701814 startingPage "2574" @default.
• W1565701814 abstract "We characterized the existence, translocation, and reabsorption during cellular activation of a constitutively expressed intracellular CD16 in the human eosinophil. By two-color flow cytometry, we showed that 6.5+/-0.3% of nonpurified eosinophils expressed surface CD16. After digestion with phosphatidylinositol-specific phospholipase C, surface CD16 on both neutrophils and eosinophils decreased substantially, suggesting that eosinophil CD16 is a glycosyl-phosphatidylinositol-linked isoform. However, CD16 was substantially expressed intracellularly in human eosinophils. Epitope-specific binding to CLB-gran11 mAb from non-NA2/NA2 donors demonstrated that intracellular eosinophil CD16 also differed from the transmembrane isoform of CD16 expressed on NK cells or macrophages. Western blot analysis performed with 3G8 or DJ130c mAb showed a broad band at approximately 65 to 80 kDa, which was the same as neutrophil CD16 from the same NA2/NA2 donors. Upon stimulation by chemoattractants C5a, FMLP, or platelet-activating-factor, eosinophilic intracellular CD16 was rapidly translocated to the eosinophil surface, expressed maximally at 30 s, and then gradually disappeared from the cell surface during the next 10 min. Intracellular flow cytometry of stimulated eosinophils and sandwich ELISA of stimulated eosinophil supernatants demonstrated that the disappearance was due to its rapid release into medium and reabsorption by the cells. Our data identify a CD16B that is consistently expressed intracellularly but only rarely on the surface of nonactivated human eosinophils. This CD16 is transiently expressed during stimulation by chemoattractants." @default.
• W1565701814 created "2016-06-24" @default.
• W1565701814 creator A5022536400 @default.
• W1565701814 creator A5037112253 @default.
• W1565701814 creator A5042985527 @default.
• W1565701814 creator A5063905122 @default.
• W1565701814 creator A5065383108 @default.
• W1565701814 creator A5076664865 @default.
• W1565701814 creator A5088949751 @default.
• W1565701814 date "1998-09-01" @default.
• W1565701814 modified "2023-09-23" @default.
• W1565701814 title "Intracellular expression of Fc gamma RIII (CD16) and its mobilization by chemoattractants in human eosinophils." @default.
• W1565701814 cites W1490976899 @default.
• W1565701814 cites W1496710275 @default.
• W1565701814 cites W1516752435 @default.
• W1565701814 cites W1520845831 @default.
• W1565701814 cites W1524240755 @default.
• W1565701814 cites W1531989373 @default.
• W1565701814 cites W1572747961 @default.
• W1565701814 cites W1593664959 @default.
• W1565701814 cites W1898483334 @default.
• W1565701814 cites W1912167037 @default.
• W1565701814 cites W1927944122 @default.
• W1565701814 cites W1928114795 @default.
• W1565701814 cites W1972394888 @default.
• W1565701814 cites W1973436145 @default.
• W1565701814 cites W1980084709 @default.
• W1565701814 cites W1999645268 @default.
• W1565701814 cites W2009811622 @default.
• W1565701814 cites W2016867245 @default.
• W1565701814 cites W2034978654 @default.
• W1565701814 cites W2041110206 @default.
• W1565701814 cites W2041794561 @default.
• W1565701814 cites W2070469182 @default.
• W1565701814 cites W2081692065 @default.
• W1565701814 cites W2084106813 @default.
• W1565701814 cites W2100837269 @default.
• W1565701814 cites W2109162598 @default.
• W1565701814 cites W2118205899 @default.
• W1565701814 cites W2129840120 @default.
• W1565701814 cites W2133762977 @default.
• W1565701814 cites W2136434117 @default.
• W1565701814 cites W2156000670 @default.
• W1565701814 cites W2156009534 @default.
• W1565701814 cites W2159064199 @default.
• W1565701814 cites W2182452189 @default.
• W1565701814 cites W2345776756 @default.
• W1565701814 cites W2403738372 @default.
• W1565701814 cites W2411322476 @default.
• W1565701814 cites W2412076249 @default.
• W1565701814 cites W2416473027 @default.
• W1565701814 cites W2418712387 @default.
• W1565701814 cites W2403519401 @default.
• W1565701814 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/9725258" @default.
• W1565701814 hasPublicationYear "1998" @default.
• W1565701814 type Work @default.
• W1565701814 sameAs 1565701814 @default.
• W1565701814 citedByCount "12" @default.
• W1565701814 countsByYear W15657018142013 @default.
• W1565701814 countsByYear W15657018142014 @default.
• W1565701814 countsByYear W15657018142018 @default.
• W1565701814 countsByYear W15657018142019 @default.
• W1565701814 countsByYear W15657018142021 @default.
• W1565701814 crossrefType "journal-article" @default.
• W1565701814 hasAuthorship W1565701814A5022536400 @default.
• W1565701814 hasAuthorship W1565701814A5037112253 @default.
• W1565701814 hasAuthorship W1565701814A5042985527 @default.
• W1565701814 hasAuthorship W1565701814A5063905122 @default.
• W1565701814 hasAuthorship W1565701814A5065383108 @default.
• W1565701814 hasAuthorship W1565701814A5076664865 @default.
• W1565701814 hasAuthorship W1565701814A5088949751 @default.
• W1565701814 hasConcept C114684123 @default.
• W1565701814 hasConcept C147483822 @default.
• W1565701814 hasConcept C153911025 @default.
• W1565701814 hasConcept C167672396 @default.
• W1565701814 hasConcept C185592680 @default.
• W1565701814 hasConcept C203014093 @default.
• W1565701814 hasConcept C2776042228 @default.
• W1565701814 hasConcept C2777037409 @default.
• W1565701814 hasConcept C2780610907 @default.
• W1565701814 hasConcept C2781462264 @default.
• W1565701814 hasConcept C553184892 @default.
• W1565701814 hasConcept C62478195 @default.
• W1565701814 hasConcept C79879829 @default.
• W1565701814 hasConcept C86803240 @default.
• W1565701814 hasConcept C95444343 @default.
• W1565701814 hasConceptScore W1565701814C114684123 @default.
• W1565701814 hasConceptScore W1565701814C147483822 @default.
• W1565701814 hasConceptScore W1565701814C153911025 @default.
• W1565701814 hasConceptScore W1565701814C167672396 @default.
• W1565701814 hasConceptScore W1565701814C185592680 @default.
• W1565701814 hasConceptScore W1565701814C203014093 @default.
• W1565701814 hasConceptScore W1565701814C2776042228 @default.
• W1565701814 hasConceptScore W1565701814C2777037409 @default.
• W1565701814 hasConceptScore W1565701814C2780610907 @default.
• W1565701814 hasConceptScore W1565701814C2781462264 @default.
• W1565701814 hasConceptScore W1565701814C553184892 @default.
• W1565701814 hasConceptScore W1565701814C62478195 @default.

• next