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• W1565798843 abstract "Since we demonstrated that qualitative differences in chromatin were related to the differences of RNA populations in different tissues and adduced evidence that the non-histone proteins of chromatin were instrumental in ‘unmasking’ specific DNA sequences, much evidence has accumulated to support this view. However, the criteria of low C 0 t RNA/DNA hybridization, which were the best available until recently, left considerable uncertainty in interpretation of results because they provided information about repetitive sequences only. We have now shown that, in the mouse, the globin genes (which occur in the unique DNA fraction) are accessible to transcription by exogenous polymerase in chromatin from erythroid tissue but not from brain tissue. In these experiments, the RNA transcribed from isolated chromatin was titrated against complementary DNA, complementary to globin mRNA and copied from it by reverse transcript-ase. We have also shown that the specificity of brain chromatin can be altered by reconstituting it in the presence of non-histone protein from erythroid tissue to make the globin genes accessible to exogenous RNA polymerase. A model for the transcriptional unit in mammalian chromatin has been proposed and will be discussed in the light of some recent observations." @default.
• W1565798843 created "2016-06-24" @default.
• W1565798843 creator A5037036496 @default.
• W1565798843 creator A5052796005 @default.
• W1565798843 date "1975-01-01" @default.
• W1565798843 modified "2023-10-02" @default.
• W1565798843 title "The Regulatory Role of Non-Histone Proteins in RNA Synthesis" @default.
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• W1565798843 doi "https://doi.org/10.1002/9780470720103.ch11" @default.
• W1565798843 hasPublicationYear "1975" @default.
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