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- W1566121179 abstract "Abstract Thin-layer (TLC) and high-performance liquid chromatographic (HPLC) methods have been developed for the separation of bis-quaternary amino steroids. The separations were performed on a silica layer and in a column, respectively. A mixture of methanol and acetonitrile was used as the organic solvents in the eluent, which contained concentrated ammonia, ammonium chloride and ammonium carbonate. In the HPLC experiments the effects of ammonium chloride and ammonium carbonate concentrations and the nature and concentration of the organic solvents in the eluents on the retentions of the compounds and separation efficiency were studied in detail. The HPLC separation system could be transformed into a TLC system using an HPTLC silica layer and a slightly modified eluent mixture, resulting in a similarly effective separation of the compounds with the same elution order. The chromatogram was evaluated by densitometry after colour reaction of the separated spots." @default.
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- W1566121179 date "1985-01-01" @default.
- W1566121179 modified "2023-09-27" @default.
- W1566121179 title "Chromatography of bis-quaternary amino steroids" @default.
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- W1566121179 doi "https://doi.org/10.1016/s0021-9673(01)87398-2" @default.
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