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- W156619880 abstract "Research Article15 March 1996free access Cytoplasmic retention and nuclear import of 5S ribosomal RNA containing RNPs. F. Rudt F. Rudt Institut für Biochemie und Molekulare Zellbiologie, Göttingen, Germany. Search for more papers by this author T. Pieler T. Pieler Institut für Biochemie und Molekulare Zellbiologie, Göttingen, Germany. Search for more papers by this author F. Rudt F. Rudt Institut für Biochemie und Molekulare Zellbiologie, Göttingen, Germany. Search for more papers by this author T. Pieler T. Pieler Institut für Biochemie und Molekulare Zellbiologie, Göttingen, Germany. Search for more papers by this author Author Information F. Rudt1 and T. Pieler1 1Institut für Biochemie und Molekulare Zellbiologie, Göttingen, Germany. The EMBO Journal (1996)15:1383-1391https://doi.org/10.1002/j.1460-2075.1996.tb00480.x PDFDownload PDF of article text and main figures. ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinked InMendeleyWechatReddit Figures & Info Nuclear export of newly transcribed 5S ribosomal RNA in Xenopus oocytes occurs in the context of either a complex with the ribosomal protein L5 (5S RNP) or with the transcription factor IIIA (7S RNP). Here we examine nuclear import of 5S RNA, L5 and TFIIIA. The 5S RNP shuttles between nucleus and cytoplasm and only 5S RNA variants which can bind to L5 gain access to the nucleus. The 7S RNP is retained in the cytoplasm. Only TFIIIA which is not bound to 5S RNA is imported into the nucleus. As a novel mechanism for cytoplasmic retention, we propose that RNA binding masks a nuclear localization sequence in TFIIIA. In contrast to the nuclear import of L5, import of TFIIIA is sensitive towards the nuclear localization sequence (NLS) competitor p(lys)-BSA, suggesting that these two proteins make use of different import pathways. Previous ArticleNext Article Volume 15Issue 61 March 1996In this issue RelatedDetailsLoading ..." @default.
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- W156619880 title "Cytoplasmic retention and nuclear import of 5S ribosomal RNA containing RNPs." @default.
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- W156619880 doi "https://doi.org/10.1002/j.1460-2075.1996.tb00480.x" @default.
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