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- W1566201148 abstract "Publisher Summary The chapter discusses methods and concepts that facilitate the detection and identification of single molecules of mRNA in situ using fluorescence in situ hybridization (FISH). FISH is a very widely used technique in cell biology. The sensitivity of detection is the major concern when implementing the FISH technology. The methodology employs stringent imaging requirements that include a carefully calibrated quantitative epifluorescence digital imaging microscope, three-dimensional (3D) optical sectioning, constrained iterative deconvolution, and 3D interactive analysis software. The analysis of point sources representing one hybridized probe has provided additional information concerning the nature of the imaging process. The pixel does not suffice as the unit for analysis of a digital image involving fluorescence in situ hybridization. The detailed characterization of biological molecules in situ is now possible using FISH. Unprecedented detail can be revealed from a hybridized cell that has been interrogated with carefully engineered, quantitatively accurate probes, followed by three-dimensional imaging and deconvolution." @default.
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- W1566201148 date "2003-01-01" @default.
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- W1566201148 title "[13] Visualization of single molecules of mRNAin Situ" @default.
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- W1566201148 doi "https://doi.org/10.1016/s0076-6879(03)61015-3" @default.
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