Matches in SemOpenAlex for { <https://semopenalex.org/work/W1566213008> ?p ?o ?g. }
- W1566213008 endingPage "206" @default.
- W1566213008 startingPage "175" @default.
- W1566213008 abstract "This chapter determines the physiological status of individual bacterial cells. are used to estimate the physiological status of individual cells in marine and estuarine water samples. (1)The Vital Stain and Probe (VSP) method is an epifluorescent direct microscopy counting technique that combines the general DNA stain DAPI, the vital stain propidium iodide (PI), and 16S rRNA targeted oligonucleotide probes to identify bacterial cells in situ. (2) Nucleoid staining technique in which the nucleoid is observed with epifluorescence microscopy when it is stained with a DNA fluorochrome such as DAPI. (3) Microautoradiography is a technique that detects the cellular localization of a radiolabeled substance ill situ. This method combines microautoradiography with 16S ribosomal RNA-targeted oligonucleotide probes, which allows simultaneous in situ identification and determination of substrate uptake patterns of individual cells. (4) Live/dead baclighttm bacterial viability kit provides a two-color fluorescent assay of bacterial viability. The kit utilizes mixtures of two nucleic acid stains: SYTO 9 and Propidium Iodide (PI). It is a membrane-impermeant stain that can passively diffuse through a cell wall and act as an indicator of loss in membrane integrity and thus as an indicator of cell viability. (5) Direct viable counting [DVC]: Kogure method utilizes the antibiotic nalidixic acid to interfere with normal cell division. In the presence of this antibiotic, sensitive cells grow but do not divide and can therefore be easily identified microscopically as elongated or enlarged cells." @default.
- W1566213008 created "2016-06-24" @default.
- W1566213008 creator A5023253387 @default.
- W1566213008 creator A5029600940 @default.
- W1566213008 creator A5034080344 @default.
- W1566213008 date "2001-01-01" @default.
- W1566213008 modified "2023-09-24" @default.
- W1566213008 title "Determining the physiological status of individual bacterial cells" @default.
- W1566213008 cites W1515985097 @default.
- W1566213008 cites W1520307276 @default.
- W1566213008 cites W1539408054 @default.
- W1566213008 cites W1580255432 @default.
- W1566213008 cites W1581285101 @default.
- W1566213008 cites W1601923928 @default.
- W1566213008 cites W1685497278 @default.
- W1566213008 cites W1691375843 @default.
- W1566213008 cites W1770972723 @default.
- W1566213008 cites W1854210985 @default.
- W1566213008 cites W1859696357 @default.
- W1566213008 cites W1919954453 @default.
- W1566213008 cites W1949287816 @default.
- W1566213008 cites W1964597224 @default.
- W1566213008 cites W1966628933 @default.
- W1566213008 cites W1980381675 @default.
- W1566213008 cites W1985787317 @default.
- W1566213008 cites W2007236728 @default.
- W1566213008 cites W2016507837 @default.
- W1566213008 cites W2016538646 @default.
- W1566213008 cites W2020245377 @default.
- W1566213008 cites W2022164804 @default.
- W1566213008 cites W2022298315 @default.
- W1566213008 cites W2026337698 @default.
- W1566213008 cites W2030256740 @default.
- W1566213008 cites W2035744376 @default.
- W1566213008 cites W2039673594 @default.
- W1566213008 cites W2044262524 @default.
- W1566213008 cites W2045956524 @default.
- W1566213008 cites W2047216151 @default.
- W1566213008 cites W2051455541 @default.
- W1566213008 cites W2052982059 @default.
- W1566213008 cites W2062379303 @default.
- W1566213008 cites W2068920473 @default.
- W1566213008 cites W2077858769 @default.
- W1566213008 cites W2078702442 @default.
- W1566213008 cites W2080936007 @default.
- W1566213008 cites W2085436481 @default.
- W1566213008 cites W2089081356 @default.
- W1566213008 cites W2090232842 @default.
- W1566213008 cites W2094569713 @default.
- W1566213008 cites W2102138046 @default.
- W1566213008 cites W2103752407 @default.
- W1566213008 cites W2104237386 @default.
- W1566213008 cites W2107828561 @default.
- W1566213008 cites W2136744552 @default.
- W1566213008 cites W2139666883 @default.
- W1566213008 cites W2147190270 @default.
- W1566213008 cites W2151472892 @default.
- W1566213008 cites W2152097062 @default.
- W1566213008 cites W2154603697 @default.
- W1566213008 cites W2155787777 @default.
- W1566213008 cites W2155927856 @default.
- W1566213008 cites W2164702895 @default.
- W1566213008 cites W2170998876 @default.
- W1566213008 cites W2474869528 @default.
- W1566213008 cites W4240190956 @default.
- W1566213008 cites W4248176371 @default.
- W1566213008 doi "https://doi.org/10.1016/s0580-9517(01)30045-4" @default.
- W1566213008 hasPublicationYear "2001" @default.
- W1566213008 type Work @default.
- W1566213008 sameAs 1566213008 @default.
- W1566213008 citedByCount "17" @default.
- W1566213008 countsByYear W15662130082022 @default.
- W1566213008 crossrefType "book-chapter" @default.
- W1566213008 hasAuthorship W1566213008A5023253387 @default.
- W1566213008 hasAuthorship W1566213008A5029600940 @default.
- W1566213008 hasAuthorship W1566213008A5034080344 @default.
- W1566213008 hasConcept C104317684 @default.
- W1566213008 hasConcept C121332964 @default.
- W1566213008 hasConcept C1491633281 @default.
- W1566213008 hasConcept C152978389 @default.
- W1566213008 hasConcept C153911025 @default.
- W1566213008 hasConcept C169274487 @default.
- W1566213008 hasConcept C190283241 @default.
- W1566213008 hasConcept C19655278 @default.
- W1566213008 hasConcept C24107716 @default.
- W1566213008 hasConcept C2775934118 @default.
- W1566213008 hasConcept C2777527632 @default.
- W1566213008 hasConcept C2781294515 @default.
- W1566213008 hasConcept C31573885 @default.
- W1566213008 hasConcept C42062724 @default.
- W1566213008 hasConcept C523546767 @default.
- W1566213008 hasConcept C53642103 @default.
- W1566213008 hasConcept C54355233 @default.
- W1566213008 hasConcept C547475151 @default.
- W1566213008 hasConcept C55493867 @default.
- W1566213008 hasConcept C62520636 @default.
- W1566213008 hasConcept C74864618 @default.
- W1566213008 hasConcept C86803240 @default.