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• W1566236542 abstract "Research Article1 December 1986free access Kinetics of endosome acidification detected by mutant and wild-type Semliki Forest virus. M.C. Kielian M.C. Kielian Search for more papers by this author M. Marsh M. Marsh Search for more papers by this author A. Helenius A. Helenius Search for more papers by this author M.C. Kielian M.C. Kielian Search for more papers by this author M. Marsh M. Marsh Search for more papers by this author A. Helenius A. Helenius Search for more papers by this author Author Information M.C. Kielian, M. Marsh and A. Helenius The EMBO Journal (1986)5:3103-3109https://doi.org/10.1002/j.1460-2075.1986.tb04616.x PDFDownload PDF of article text and main figures. ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinked InMendeleyWechatReddit Figures & Info The fusogenic properties of Semliki Forest virus (SFV) and its mutants were used to follow the kinetics of acidification during the endocytic uptake of virus by BHK-21 cells. It has previously been shown that the low pH of endocytic vacuoles triggers a conformational change in the SFV spike glycoprotein, activating membrane fusion and initiating virus infection. This conformational alteration was here shown to occur in endosomes and to follow the same time course as the intracellular fusion reaction, demonstrating that fusion occurs rapidly after virus exposure to endosome acidity. The kinetics of endosome acidification were monitored using wild type (wt) SFV and fus-1, an SFV mutant with a lower fusion pH threshold. The results presented here demonstrated that wt and mutant virus were internalized with a t1/2 of 10 min, and that endosomes were acidified to the wt threshold of pH 6.2 with a t1/2 of 15 min. In contrast, endosome pH reached the fus-1 threshold of 5.3 with a much longer t1/2 of 45 min. The subsequent degradation of SFV in lysosomes had a t1/2 of 90 min. It was found that after the initial uptake of virus from the plasma membrane, its transit through the endocytic pathway, exposure to endosome acidity and eventual delivery to lysosomes were markedly asynchronous. Previous ArticleNext Article Volume 5Issue 121 December 1986In this issue RelatedDetailsLoading ..." @default.
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• W1566236542 date "1986-12-01" @default.
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• W1566236542 title "Kinetics of endosome acidification detected by mutant and wild-type Semliki Forest virus." @default.
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• W1566236542 doi "https://doi.org/10.1002/j.1460-2075.1986.tb04616.x" @default.
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