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- W1566303947 abstract "A starter strain, Lactobacillus casei C257, was found to carry a lactose plasmid, pLY101. Restriction mapping showed that pLY101 DNA was 68.2 kilobases long. Since a non-lactose-utilizing variant of C257, MSK248, lost phospho-β-galactosidase (P-β-gal) activity and pLY101 DNA had a sequence(s) homologous to the streptococcal fragment including a P-β-gal gene, pLY101 is likely to encode a P-β-gal gene required for lactose metabolism in C257. MSK248 grew in galactose medium at a rate identical to that of C257 and retained phosphoenolpyruvate-dependent phosphotransferase system activity for lactose similar to that of C257. Therefore, the C257 chromosome appears to encode a complete set of genes for the lactose-phosphotransferase system and the predominant galactose metabolic pathway in C257. pLY101 DNA had a sequence homologous to a lactobacillus insertion sequence, ISL 1 , which mapped more than 12 kilobases from the sequence homologous to the streptococcal P-β-gal fragment." @default.
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- W1566303947 date "1987-12-01" @default.
- W1566303947 modified "2023-09-24" @default.
- W1566303947 title "Properties of Lactose Plasmid pLY101 in <i>Lactobacillus casei</i>" @default.
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- W1566303947 doi "https://doi.org/10.1128/aem.53.12.2987-2991.1987" @default.
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