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- W1566335920 abstract "Abstract The distribution of polypyrimidine sequences has been studied in 28 S rRNA from Novikoff hepatoma ascites cells. Oligonucleotides were released from 32P-labeled RNA by successive digestion with U2 RNase followed by T1 RNase. The various fragments were initially separated by chromatography according to chain length on DEAE-Sephadex A-25 at pH 7.5. The fragments in each isostich were further separated according to base composition by electrophoresis on cellulose acetate strips or Whatman No. 3MM paper at pH 3.5. On the basis of electrophoretic mobility and nucleotide composition, 118 fragments were found with chain lengths of 5 to 21, including 55 pentanucleotides, 26 hexanucleotides, 11 heptanucleotides, 8 octanucleotides, 5 nonanucleotides, 3 decanucleotides, 2 undecanucleotides, 1 dodecanucleotide, 2 tridecanucleotides, 1 tetradecanucleotide, 2 pentadecanucleotides, 1 hexadecanucleotide, and 1 unicosanucleotide. The sequences of 94 of these containing 667 nucleotides are presented, the largest being an unicosanucleotide (U-U-C-C-C-C-C-C-U-C-U-C-C-U-C-U-U-C-C-C-Gp). On the basis of electrophoretic mobility and nucleotide composition, 24 of the fragments of chain length 6 or greater are specific markers for 28 S rRNA and not present in 18 S rRNA. Furthermore, only 8 of the 62 deduced sequences of chain length 6 or greater are common to 18 S rRNA. Eight alkali-stable markers in 28 S rRNA were isolated in polypyrimidine oligonucleotides including a trinucleotide, Um-Gm-Up, and a tetranucleotide, Am-Gm-Cm-Ap. The molar yields of the marker and other fragments and the lack of contamination with marker fragments of 18 S rRNA are consistent with a high degree of homogeneity of mammalian 28 S rRNA." @default.
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- W1566335920 date "1974-02-01" @default.
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- W1566335920 title "Structural Analyses of Mammalian Ribosomal Ribonucleic Acid and Its Precursors" @default.
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- W1566335920 doi "https://doi.org/10.1016/s0021-9258(19)43019-6" @default.
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