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- W1566396150 abstract "Broilers are considered susceptible to ante-mortem stressors and their muscles are characterised by pathological damage and heat-stress is known to have its role in further exacerbation of this condition. Present study was designed to find out the role of heat-stress in muscle damage and genes involved in muscle damage. 32 microarray slides (8 broiler heat stressed, 8 broiler controls, 8 layer heat stressed, 8 layer control) were analysed in GenStat, 2214 no. of probes were found significant for Breed × Treatment interaction. These Significant probes were further analysed in BioLayout Express. Some interested clusters were selected on the basis of their respective graphs, based on expression data and their probes were analysed in Ingenuity Pathway Analysis (IPA) to get the respective canonical pathways. In these clusters, 287 genes were present which are involved in stress response, inflammation and tissue damage, on the basis of their GO terms and published literature. In all these clusters the expression levels of control broilers were higher than layers (although the layers had 0.96 0 C higher body temperature than broilers in control condition which suggest that BC were more stressed than layers) and heat-stress resulted in further exacerbation of these differences. From the pathway analysis of these probes, some significant pathways were selected on the basis of their relevance to inflammation, stress, apoptosis and tissue damage e.g. fMLP Signaling in Neutrophils, CCR3 Signaling in Eosinophils and Chemokine Signalling are involved in inflammation, increase intracellular calcium (Garcia-Rodriguez and Rao, 2000;" @default.
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- W1566396150 date "2011-01-01" @default.
- W1566396150 modified "2023-09-23" @default.
- W1566396150 title "Identification of novel pathways/genes for muscle damage in broilers" @default.
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