FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1566398478> ?p ?o ?g. }

• W1566398478 endingPage "14081" @default.
• W1566398478 startingPage "14076" @default.
• W1566398478 abstract "To elucidate the role of individual amino acid residues in stabilizing the conformation of a protein, the stabilities of wild-type tryptophan synthase alpha-subunit from Escherichia coli and seven mutant proteins substituted by single amino acid residues at position 49, which is buried in the interior of the protein, were compared. The mutant proteins have Gln, Met, Val, Tyr, Leu, Ser, or Lys in place of Glu in the wild-type protein. The dissociation constant, pK, of the Glu residue at position 49 for the wild-type protein was determined to be 7.5 from a titration curve obtained by comparison of two-dimensional isoelectric focusing electrophoresis of the wild-type and mutant proteins. Our results indicate that 1) the conformational stabilities of the proteins studied increase linearly with hydrophobicity of the substituting residues (except Tyr), with the coefficient of this linear dependence being 2.0, 3.4, or 1.3 at pH 5.5, 7.0, or 9.0, respectively; and 2) Lys or Glu at position 49 serve as a destabilizing factor when ionized." @default.
• W1566398478 created "2016-06-24" @default.
• W1566398478 creator A5028649351 @default.
• W1566398478 creator A5041279842 @default.
• W1566398478 creator A5066940666 @default.
• W1566398478 creator A5072375030 @default.
• W1566398478 creator A5078324283 @default.
• W1566398478 date "1984-11-01" @default.
• W1566398478 modified "2023-10-16" @default.
• W1566398478 title "Effect of amino acid residues on conformational stability in eight mutant proteins variously substituted at a unique position of the tryptophan synthase alpha-subunit." @default.
• W1566398478 cites W1928528182 @default.
• W1566398478 cites W1969877152 @default.
• W1566398478 cites W1978478240 @default.
• W1566398478 cites W1992073337 @default.
• W1566398478 cites W2009500272 @default.
• W1566398478 cites W2016303898 @default.
• W1566398478 cites W2023692875 @default.
• W1566398478 cites W2032846506 @default.
• W1566398478 cites W2034973897 @default.
• W1566398478 cites W2051425578 @default.
• W1566398478 cites W2053668583 @default.
• W1566398478 cites W2060513801 @default.
• W1566398478 cites W2092045508 @default.
• W1566398478 cites W2101987873 @default.
• W1566398478 cites W2108539611 @default.
• W1566398478 cites W2114441129 @default.
• W1566398478 cites W2137476041 @default.
• W1566398478 cites W2314111724 @default.
• W1566398478 cites W2406165452 @default.
• W1566398478 cites W268238884 @default.
• W1566398478 cites W4301267713 @default.
• W1566398478 cites W809571466 @default.
• W1566398478 cites W998400604 @default.
• W1566398478 doi "https://doi.org/10.1016/s0021-9258(18)89858-1" @default.
• W1566398478 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/6389541" @default.
• W1566398478 hasPublicationYear "1984" @default.
• W1566398478 type Work @default.
• W1566398478 sameAs 1566398478 @default.
• W1566398478 citedByCount "47" @default.
• W1566398478 crossrefType "journal-article" @default.
• W1566398478 hasAuthorship W1566398478A5028649351 @default.
• W1566398478 hasAuthorship W1566398478A5041279842 @default.
• W1566398478 hasAuthorship W1566398478A5066940666 @default.
• W1566398478 hasAuthorship W1566398478A5072375030 @default.
• W1566398478 hasAuthorship W1566398478A5078324283 @default.
• W1566398478 hasBestOaLocation W15663984781 @default.
• W1566398478 hasConcept C104292427 @default.
• W1566398478 hasConcept C104317684 @default.
• W1566398478 hasConcept C112243037 @default.
• W1566398478 hasConcept C143065580 @default.
• W1566398478 hasConcept C159110408 @default.
• W1566398478 hasConcept C181199279 @default.
• W1566398478 hasConcept C185592680 @default.
• W1566398478 hasConcept C2775944032 @default.
• W1566398478 hasConcept C2776706248 @default.
• W1566398478 hasConcept C2779882130 @default.
• W1566398478 hasConcept C49453240 @default.
• W1566398478 hasConcept C515207424 @default.
• W1566398478 hasConcept C55493867 @default.
• W1566398478 hasConcept C64943373 @default.
• W1566398478 hasConcept C71240020 @default.
• W1566398478 hasConcept C71924100 @default.
• W1566398478 hasConcept C83730767 @default.
• W1566398478 hasConceptScore W1566398478C104292427 @default.
• W1566398478 hasConceptScore W1566398478C104317684 @default.
• W1566398478 hasConceptScore W1566398478C112243037 @default.
• W1566398478 hasConceptScore W1566398478C143065580 @default.
• W1566398478 hasConceptScore W1566398478C159110408 @default.
• W1566398478 hasConceptScore W1566398478C181199279 @default.
• W1566398478 hasConceptScore W1566398478C185592680 @default.
• W1566398478 hasConceptScore W1566398478C2775944032 @default.
• W1566398478 hasConceptScore W1566398478C2776706248 @default.
• W1566398478 hasConceptScore W1566398478C2779882130 @default.
• W1566398478 hasConceptScore W1566398478C49453240 @default.
• W1566398478 hasConceptScore W1566398478C515207424 @default.
• W1566398478 hasConceptScore W1566398478C55493867 @default.
• W1566398478 hasConceptScore W1566398478C64943373 @default.
• W1566398478 hasConceptScore W1566398478C71240020 @default.
• W1566398478 hasConceptScore W1566398478C71924100 @default.
• W1566398478 hasConceptScore W1566398478C83730767 @default.
• W1566398478 hasIssue "22" @default.
• W1566398478 hasLocation W15663984781 @default.
• W1566398478 hasOpenAccess W1566398478 @default.
• W1566398478 hasPrimaryLocation W15663984781 @default.
• W1566398478 hasRelatedWork W1494353014 @default.
• W1566398478 hasRelatedWork W1566398478 @default.
• W1566398478 hasRelatedWork W1663202091 @default.
• W1566398478 hasRelatedWork W1982348788 @default.
• W1566398478 hasRelatedWork W2000978079 @default.
• W1566398478 hasRelatedWork W2036606630 @default.
• W1566398478 hasRelatedWork W2070951571 @default.
• W1566398478 hasRelatedWork W2091945947 @default.
• W1566398478 hasRelatedWork W3106880946 @default.
• W1566398478 hasRelatedWork W99077715 @default.
• W1566398478 hasVolume "259" @default.
• W1566398478 isParatext "false" @default.
• W1566398478 isRetracted "false" @default.
• W1566398478 magId "1566398478" @default.

• next