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- W1566440650 abstract "Immunoglobulin (Ig) b4 allotypic determinants are detected on the surface membrane of rabbit peripheral blood lymphocytes by an indirect immunoferritin labeling technique. Cells coated with antiallotype antibodies are labelled with soluble complexes of ferritin and rabbit antiferritin of a given allotype. At 0 °C a patchy distribution of labeled surface immunoglobulin is visualized on 80% of the lymphocytes examined. Warming of the cells for 1–5 min at 37 °C causes rapid endocytosis of surface label in a perinuclear fashion. Cap formation is not observed. Cross-linking of immunoferritin labelled surface determinants with sheep anti-rabbit Ig (SARG) inhibits endocytosis and promotes aggregation of small surface patches. Indirect evidence suggests that sloughing and/or stripping of labelled surface Ig can occur after this aggregation. These surface changes may be the first step in the induction of lymphocyte activation." @default.
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- W1566440650 date "1974-06-01" @default.
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- W1566440650 title "Surface immunoglobulin on rabbit lymphoid cells" @default.
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- W1566440650 doi "https://doi.org/10.1016/0008-8749(74)90100-2" @default.
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