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- W1566452574 abstract "Although gene sequence data for parasitic nematodes have increased significantly in the last decade, there is currently no reliable method to test gene function and validate putative control targets. Gene silencing by RNA interference (RNAi) has proven successful in the free-living nematode Caenorhabditis elegans and the ease of this technique has allowed it to be adapted for high-throughput genome-wide screening of essential gene function. This success encouraged a number of RNAi studies on parasitic nematodes of animals and plants. Whereas RNAi in parasitic nematodes, as well as some free-living nematodes, is not as effective as it is in C. elegans, specific gene silencing has been observed for some target genes. Recent work with Haemonchus contortus suggests that the success of RNAi can be improved by targeting genes expressed in sites accessible to the environment such as the intestine, excretory/secretory system, or amphids. One of the genes robustly silenced in H. contortus encodes the aminopeptidase H11. RNAi of H11 in larvae prior to oral infection resulted in significant reductions in worm burden and egg output, similar to vaccination with native H11 protein. This provides proof-of-principle that RNAi can be developed as a tool to identify essential gene function. Using current RNAi techniques, screening for essential genes as potential control targets may be limited by transcript accessibility to introduced double-stranded RNA. Optimization of double-stranded RNA delivery methods is discussed as enhanced uptake may allow RNAi to be fully exploited as a tool for both functional genomics and target discovery in parasitic nematodes." @default.
- W1566452574 created "2016-06-24" @default.
- W1566452574 creator A5089634063 @default.
- W1566452574 date "2012-07-18" @default.
- W1566452574 modified "2023-09-25" @default.
- W1566452574 title "RNA Interference: A Potential Discovery Tool for Therapeutic Targets of Parasitic Nematodes" @default.
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- W1566452574 doi "https://doi.org/10.1002/9783527652969.ch6" @default.
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