FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1566647207> ?p ?o ?g. }

• W1566647207 endingPage "495" @default.
• W1566647207 startingPage "474" @default.
• W1566647207 abstract "A collection of tagged deletion mutant strains was created in Streptococcus mutans UA159 to facilitate investigation of the aciduric capability of this oral pathogen. Gene-specific barcoded deletions were attempted in 1432 open reading frames (representing 73% of the genome), and resulted in the isolation of 1112 strains (56% coverage) carrying deletions in distinct non-essential genes. As S. mutans virulence is predicated upon the ability of the organism to survive an acidic pH environment, form biofilms on tooth surfaces, and out-compete other oral microflora, we assayed individual mutant strains for the relative fitness of the deletion strain, compared with the parent strain, under acidic and oxidative stress conditions, as well as for their ability to form biofilms in glucose- or sucrose-containing medium. Our studies revealed a total of 51 deletion strains with defects in both aciduricity and biofilm formation. We have also identified 49 strains whose gene deletion confers sensitivity to oxidative damage and deficiencies in biofilm formation. We demonstrate the ability to examine competitive fitness of mutant organisms using the barcode tags incorporated into each deletion strain to examine the representation of a particular strain in a population. Co-cultures of deletion strains were grown either in vitro in a chemostat to steady-state values of pH 7 and pH 5 or in vivo in an animal model for oral infection. Taken together, these data represent a mechanism for assessing the virulence capacity of this pathogenic microorganism and a resource for identifying future targets for drug intervention to promote healthy oral microflora." @default.
• W1566647207 created "2016-06-24" @default.
• W1566647207 creator A5001584398 @default.
• W1566647207 creator A5002625880 @default.
• W1566647207 creator A5006594303 @default.
• W1566647207 creator A5017473873 @default.
• W1566647207 creator A5024145753 @default.
• W1566647207 creator A5031825394 @default.
• W1566647207 creator A5046373482 @default.
• W1566647207 creator A5052015542 @default.
• W1566647207 creator A5054709999 @default.
• W1566647207 creator A5057313766 @default.
• W1566647207 creator A5071586067 @default.
• W1566647207 creator A5073402912 @default.
• W1566647207 creator A5086370550 @default.
• W1566647207 date "2015-06-19" @default.
• W1566647207 modified "2023-10-17" @default.
• W1566647207 title "Functional profiling in<i>Streptococcus mutans</i>: construction and examination of a genomic collection of gene deletion mutants" @default.
• W1566647207 cites W1499500424 @default.
• W1566647207 cites W1853023758 @default.
• W1566647207 cites W1881346788 @default.
• W1566647207 cites W1899848340 @default.
• W1566647207 cites W1963651147 @default.
• W1566647207 cites W1965605504 @default.
• W1566647207 cites W1968038293 @default.
• W1566647207 cites W1971814245 @default.
• W1566647207 cites W1977782657 @default.
• W1566647207 cites W1979338499 @default.
• W1566647207 cites W1983351627 @default.
• W1566647207 cites W1987144692 @default.
• W1566647207 cites W1987427453 @default.
• W1566647207 cites W1989573570 @default.
• W1566647207 cites W1990814944 @default.
• W1566647207 cites W1999653658 @default.
• W1566647207 cites W2003491532 @default.
• W1566647207 cites W2005083819 @default.
• W1566647207 cites W2005172648 @default.
• W1566647207 cites W2007380246 @default.
• W1566647207 cites W2018189646 @default.
• W1566647207 cites W2039214433 @default.
• W1566647207 cites W2040670736 @default.
• W1566647207 cites W2048152156 @default.
• W1566647207 cites W2048593866 @default.
• W1566647207 cites W2057331790 @default.
• W1566647207 cites W2057485750 @default.
• W1566647207 cites W2058975831 @default.
• W1566647207 cites W2060481943 @default.
• W1566647207 cites W2074819592 @default.
• W1566647207 cites W2077575442 @default.
• W1566647207 cites W2094285560 @default.
• W1566647207 cites W2100478279 @default.
• W1566647207 cites W2101587791 @default.
• W1566647207 cites W2103686288 @default.
• W1566647207 cites W2104246257 @default.
• W1566647207 cites W2104771379 @default.
• W1566647207 cites W2105240801 @default.
• W1566647207 cites W2107757630 @default.
• W1566647207 cites W2107796633 @default.
• W1566647207 cites W2110591870 @default.
• W1566647207 cites W2127113854 @default.
• W1566647207 cites W2128301757 @default.
• W1566647207 cites W2130338082 @default.
• W1566647207 cites W2133491210 @default.
• W1566647207 cites W2135102440 @default.
• W1566647207 cites W2136936386 @default.
• W1566647207 cites W2139119322 @default.
• W1566647207 cites W2139962100 @default.
• W1566647207 cites W2142437744 @default.
• W1566647207 cites W2143645881 @default.
• W1566647207 cites W2144388403 @default.
• W1566647207 cites W2146264532 @default.
• W1566647207 cites W2146459311 @default.
• W1566647207 cites W2146554466 @default.
• W1566647207 cites W2163481720 @default.
• W1566647207 cites W2164028009 @default.
• W1566647207 cites W2167187012 @default.
• W1566647207 cites W2406460042 @default.
• W1566647207 cites W2523457784 @default.
• W1566647207 cites W4240062648 @default.
• W1566647207 doi "https://doi.org/10.1111/omi.12107" @default.
• W1566647207 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4636983" @default.
• W1566647207 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/25973955" @default.
• W1566647207 hasPublicationYear "2015" @default.
• W1566647207 type Work @default.
• W1566647207 sameAs 1566647207 @default.
• W1566647207 citedByCount "34" @default.
• W1566647207 countsByYear W15666472072015 @default.
• W1566647207 countsByYear W15666472072016 @default.
• W1566647207 countsByYear W15666472072017 @default.
• W1566647207 countsByYear W15666472072018 @default.
• W1566647207 countsByYear W15666472072019 @default.
• W1566647207 countsByYear W15666472072020 @default.
• W1566647207 countsByYear W15666472072021 @default.
• W1566647207 countsByYear W15666472072022 @default.
• W1566647207 countsByYear W15666472072023 @default.
• W1566647207 crossrefType "journal-article" @default.
• W1566647207 hasAuthorship W1566647207A5001584398 @default.
• W1566647207 hasAuthorship W1566647207A5002625880 @default.

• next