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- W1566684085 abstract "Two types of baby-hamster kidney (BHK) cell line, anchorage-dependent BHK-An(32) and suspended BHK-An(48) cells, were cultivated on non-woven polyester fabrics (NWPF) that were prepared from non-woven polyethylene teraphthalate sheets with different void fractions in three-dimensional configuration. The attachment and growth characteristics of both cell lines were investigated in stationary and submerged cultures, containing Dulbecco's modified Eagle's medium supplemented with fetal-calf serum. All cultures displayed rapid attachment (<120 min) of inoculated cells to the discs. The growth of BHK cells on NWPF discs in stationary cultures was similar to that observed in tissue-culture dishes; however, the denser discs showed better results in terms of final cell density, especially in the case of BHK-An(48) cells. Spinner-flask cultures indicated that higher cell yields were obtained than in stationary conditions. Microscopic observation showed that anchorage-dependent BHK-An(32) cells spread through the interfibre spaces of NPWF discs to form three-dimensional structures whereas BHK-An(48) cells proliferated on fibres." @default.
- W1566684085 created "2016-06-24" @default.
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- W1566684085 date "1999-08-01" @default.
- W1566684085 modified "2023-10-18" @default.
- W1566684085 title "Anchorage-dependent and suspended baby-hamster kidney cells on three-dimensional non-woven polyester fabric discs: comparison of growth characteristics." @default.
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