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- W1566687059 abstract "Real time investigation of protein folding, structure, and dynamics at high resolution in living cells, is the next major step in nano cell biology. However, there are a number of major hurdles that need to be overcome, such as coupled translation and folding, the intervention of chaperones and folding enzymes, translocation, export, and the targeting machinery, proteasomes and degradation signals, etc. Although some progress has been made in recent years (Royer, 2006), current technology available in the field is limited for an enhanced resolution study, and no major breakthrough has been achieved in this direction. The major challenge is to develop new approaches that permit high‐resolution, direct, and accurate structural measurements of protein folding and structure, in specific compartments of living cells. In this chapter, we focus on a review of a QQ‐reagent based protein transduction recently developed in our laboratory technology that may allow us to develop a high‐resolution in vivo investigation of protein folding, structure, and dynamics." @default.
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- W1566687059 date "2008-01-01" @default.
- W1566687059 modified "2023-10-15" @default.
- W1566687059 title "Chapter 14 Real Time Investigation of Protein Folding, Structure, and Dynamics in Living Cells" @default.
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- W1566687059 doi "https://doi.org/10.1016/s0091-679x(08)00814-5" @default.
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