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- W1566744526 abstract "Abstract O smia cornifrons plays a major role in the pollination of orchards, but basic information on vitellogenin and oocyte development is limited. To better understand vitellogenin in hymenopteran insects, we cloned a c DNA encoding vitellogenin from the hornfaced bee O . cornifrons . O smia cornifrons vitellogenin c DNA contains 5477 bp with an open reading frame of 1783 amino acid residues, and has a predicted molecular mass of approximately 200.21 k D a and a p I of 6.55. O smia cornifrons vitellogenin possesses four consensus ( RXXR / S ) cleavage sites and has conserved DGXR and GL / ICG motifs in the C ‐terminus. The deduced amino acid sequence of the O . cornifrons vitellogenin c DNA showed a 66% identity with M egachile rotundata , 53% to A pis mellifera , 51% to B ombus ignitus and 42%–30% with other hymenopteran insect vitellogenins. Phylogenetic analysis showed that O . cornifrons vitellogenin clustered with vitellogenins from M egachilidae, A pidae, V espidae and F ormicidae species but not with those from P teromalidae, A phelinidae or I chneumonidae species. The expression profile of O . cornifrons vitellogenin m RNA during development revealed that O . cornifrons vitellogenin was first detected in the pupal stage and was continuously detected during the adult stage. Interestingly, O . cornifrons vitellogenin m RNA expression was low in mid‐diapause, then gradually increased beginning on day 3 of the newly emerged adult stage, and subsequently declined. These results suggest that the expression level of O . cornifrons vitellogenin m RNA is stage‐specific." @default.
- W1566744526 created "2016-06-24" @default.
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- W1566744526 date "2015-03-01" @default.
- W1566744526 modified "2023-10-05" @default.
- W1566744526 title "<i>Osmia cornifrons</i>vitellogenin: cDNA cloning, structural analysis and developmental expression" @default.
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- W1566744526 doi "https://doi.org/10.1111/1748-5967.12098" @default.
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