Matches in SemOpenAlex for { <https://semopenalex.org/work/W1566771367> ?p ?o ?g. }
- W1566771367 endingPage "26857" @default.
- W1566771367 startingPage "26848" @default.
- W1566771367 abstract "The export of vesicular stomatitis virus glycoprotein (VSV-G) from the endoplasmic reticulum (ER) involves sorting and concentration, and has been proposed to require the function of heterotrimeric G proteins. To begin to identify the basic elements of a potential signaling pathway involved in vesicle assembly, we have examined whether protein kinase C (PKC) is required for ER to Golgi transport. Calphostin C, a specific inhibitor of the highly conserved cysteine-rich C6H2 motif present in the regulatory domain of PKC was found to be a potent inhibitor of export of VSV-G and vesicle budding from the ER in vivo and in vitro (IC50 approximately 60 nM). In contrast, the diacylglycerol analog phorbol 12-myristate 13-acetate, which activates PKC, enhanced the migration of VSV-G from the ER to pre-Golgi intermediates. Neither reagent had detectable effects on the oligomerization of VSV-G prior to export nor perturbed transport of protein between compartments of the Golgi stack. In contrast to the striking effects of calphostin C, reagents that inhibit the function of the catalytic domain of PKC (including the general kinase inhibitor staurosporine, as well as the more specific inhibitors H-7, H-8, pseudosubstrate inhibitor, or chelerythrine) did not inhibit export from the ER. Export was also insensitive to down-regulation of various PKC isoforms. These results suggest that a novel protein containing the conserved C6H2 motif may serve as a potential link in a signaling pathway regulating vesicle budding from the ER." @default.
- W1566771367 created "2016-06-24" @default.
- W1566771367 creator A5027605645 @default.
- W1566771367 creator A5062435927 @default.
- W1566771367 creator A5079375370 @default.
- W1566771367 date "1994-10-01" @default.
- W1566771367 modified "2023-09-28" @default.
- W1566771367 title "Export of protein from the endoplasmic reticulum is regulated by a diacylglycerol/phorbol ester binding protein." @default.
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