Matches in SemOpenAlex for { <https://semopenalex.org/work/W1566774081> ?p ?o ?g. }
Showing items 1 to 93 of
93
with 100 items per page.
- W1566774081 endingPage "2399" @default.
- W1566774081 startingPage "2391" @default.
- W1566774081 abstract "To produce ubiquitinated substrates for studies on ATP-dependent proteolysis, 125I-lysozyme was incubated in hemin-inhibited rabbit reticulocyte lysates. A portion of the labeled molecules became linked to ubiquitin in large covalent complexes. When these were partially purified and returned to uninhibited lysates containing ATP, the conjugated lysozyme molecules were degraded 10 times faster than free lysozyme. Purification of covalently modified lysozyme from hemin-inhibited lysates containing 125I-ubiquitin and 131I-lysozyme confirmed that both molecules were present in the complexes. The doubly labeled conjugates also permitted us to determine the fate of each molecule in uninhibited lysates. Besides degradation of lysozyme, there was a progressive release of intact lysozyme molecules from the complexes. This disassembly, which was the only fate of the complexes in the absence of ATP, proceeded through a series of smaller intermediates, several having molecular weights expected for ubiquitin-lysozyme conjugates, and eventually free lysozyme was regenerated. The behavior of labeled ubiquitin was similar, though not identical, to that of lysozyme. Even in lysates containing ATP ubiquitin emerged from the complex undegraded. Furthermore, ubiquitin was present in a greater number of species than was lysozyme. The demonstration that ubiquitin-lysozyme conjugates are rapidly degraded provides support for the hypothesis of Hershko, Rose, Ciechanover, and their colleagues that a key function of ubiquitin is to modify the proteolytic substrate. Further support for the hypothesis is presented in the following paper where we show that the conjugated lysozyme molecules are substrates for an ATP-dependent protease that does not degrade free lysozyme." @default.
- W1566774081 created "2016-06-24" @default.
- W1566774081 creator A5040361591 @default.
- W1566774081 creator A5077603452 @default.
- W1566774081 date "1986-02-01" @default.
- W1566774081 modified "2023-10-05" @default.
- W1566774081 title "Ubiquitin-lysozyme conjugates. Purification and susceptibility to proteolysis." @default.
- W1566774081 cites W1502675762 @default.
- W1566774081 cites W1513849811 @default.
- W1566774081 cites W1519470329 @default.
- W1566774081 cites W1767859166 @default.
- W1566774081 cites W1967216090 @default.
- W1566774081 cites W1968229731 @default.
- W1566774081 cites W1969336855 @default.
- W1566774081 cites W1990034709 @default.
- W1566774081 cites W1991276331 @default.
- W1566774081 cites W1998475203 @default.
- W1566774081 cites W1998790265 @default.
- W1566774081 cites W2005852971 @default.
- W1566774081 cites W2009441693 @default.
- W1566774081 cites W2024220328 @default.
- W1566774081 cites W2033936124 @default.
- W1566774081 cites W2034941135 @default.
- W1566774081 cites W2051583452 @default.
- W1566774081 cites W2057700578 @default.
- W1566774081 cites W2062663235 @default.
- W1566774081 cites W2092275002 @default.
- W1566774081 cites W2100837269 @default.
- W1566774081 cites W2140182533 @default.
- W1566774081 cites W2158714866 @default.
- W1566774081 cites W2159137448 @default.
- W1566774081 cites W2159823053 @default.
- W1566774081 doi "https://doi.org/10.1016/s0021-9258(17)35949-5" @default.
- W1566774081 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/3003113" @default.
- W1566774081 hasPublicationYear "1986" @default.
- W1566774081 type Work @default.
- W1566774081 sameAs 1566774081 @default.
- W1566774081 citedByCount "100" @default.
- W1566774081 countsByYear W15667740812013 @default.
- W1566774081 countsByYear W15667740812014 @default.
- W1566774081 countsByYear W15667740812016 @default.
- W1566774081 countsByYear W15667740812019 @default.
- W1566774081 countsByYear W15667740812020 @default.
- W1566774081 countsByYear W15667740812021 @default.
- W1566774081 crossrefType "journal-article" @default.
- W1566774081 hasAuthorship W1566774081A5040361591 @default.
- W1566774081 hasAuthorship W1566774081A5077603452 @default.
- W1566774081 hasBestOaLocation W15667740811 @default.
- W1566774081 hasConcept C104317684 @default.
- W1566774081 hasConcept C134306372 @default.
- W1566774081 hasConcept C181199279 @default.
- W1566774081 hasConcept C185592680 @default.
- W1566774081 hasConcept C197336794 @default.
- W1566774081 hasConcept C25602115 @default.
- W1566774081 hasConcept C2776966407 @default.
- W1566774081 hasConcept C2781307694 @default.
- W1566774081 hasConcept C33923547 @default.
- W1566774081 hasConcept C55493867 @default.
- W1566774081 hasConcept C86803240 @default.
- W1566774081 hasConcept C95444343 @default.
- W1566774081 hasConceptScore W1566774081C104317684 @default.
- W1566774081 hasConceptScore W1566774081C134306372 @default.
- W1566774081 hasConceptScore W1566774081C181199279 @default.
- W1566774081 hasConceptScore W1566774081C185592680 @default.
- W1566774081 hasConceptScore W1566774081C197336794 @default.
- W1566774081 hasConceptScore W1566774081C25602115 @default.
- W1566774081 hasConceptScore W1566774081C2776966407 @default.
- W1566774081 hasConceptScore W1566774081C2781307694 @default.
- W1566774081 hasConceptScore W1566774081C33923547 @default.
- W1566774081 hasConceptScore W1566774081C55493867 @default.
- W1566774081 hasConceptScore W1566774081C86803240 @default.
- W1566774081 hasConceptScore W1566774081C95444343 @default.
- W1566774081 hasIssue "5" @default.
- W1566774081 hasLocation W15667740811 @default.
- W1566774081 hasOpenAccess W1566774081 @default.
- W1566774081 hasPrimaryLocation W15667740811 @default.
- W1566774081 hasRelatedWork W1964253087 @default.
- W1566774081 hasRelatedWork W1986393148 @default.
- W1566774081 hasRelatedWork W2002189973 @default.
- W1566774081 hasRelatedWork W2004754196 @default.
- W1566774081 hasRelatedWork W2043230770 @default.
- W1566774081 hasRelatedWork W2086984568 @default.
- W1566774081 hasRelatedWork W2404348892 @default.
- W1566774081 hasRelatedWork W2414415093 @default.
- W1566774081 hasRelatedWork W2949098585 @default.
- W1566774081 hasRelatedWork W3010056926 @default.
- W1566774081 hasVolume "261" @default.
- W1566774081 isParatext "false" @default.
- W1566774081 isRetracted "false" @default.
- W1566774081 magId "1566774081" @default.
- W1566774081 workType "article" @default.