Matches in SemOpenAlex for { <https://semopenalex.org/work/W1566861922> ?p ?o ?g. }
Showing items 1 to 81 of
81
with 100 items per page.
- W1566861922 endingPage "2134" @default.
- W1566861922 startingPage "2130" @default.
- W1566861922 abstract "A sensitive ESR method which allows a direct quantitative determination of nucleic acid binding affinities of proteins under physiologically relevant conditions has been applied to the gene 5 protein of bacteriophage fd. This was achieved with two spin-labeled nucleic acids, (ldT, dT)n and (lA,A)n, which served as macro-molecular spin probes in ESR competition experiments. With the two different macromolecular spin probes, it was possible to determine the relative apparent affinity constants, Kapp, over a large affinity domain. In 20 mM Tris X HCl (pH 8.1), 1 mM sodium EDTA, 0.1 mM dithiothreitol, 10% (w/v) glycerol, 0.05% Triton, and 125 mM NaCl, the following affinity relationship was observed: K(dT)napp = 10(3) KfdDNAapp = 2 X 10(4) K(A)napp = 6.6 X 10(4) KrRNAapp = 1.5 X 10(5) KR17RNAapp. Increasing the [NaCl] from 125 to 200 mM caused considerably less tight binding of gene 5 protein to (lA,A)n, and a typical cooperative binding isotherm was observed, whereas at the lower [NaCl] used for the competition experiments, the binding was essentially stoichiometric. A computer fit of the experimental titration data at 200 mM NaCl gave an intrinsic binding constant, Kint, of 1300 M-1 and a cooperativity factor, omega, of 60 (Kint omega = Kapp) for (lA,A)n." @default.
- W1566861922 created "2016-06-24" @default.
- W1566861922 creator A5000872039 @default.
- W1566861922 creator A5050920574 @default.
- W1566861922 creator A5020813055 @default.
- W1566861922 date "1984-02-01" @default.
- W1566861922 modified "2023-09-30" @default.
- W1566861922 title "Nucleic acid binding affinity of fd gene 5 protein in the cooperative binding mode." @default.
- W1566861922 cites W1537704738 @default.
- W1566861922 cites W1597227895 @default.
- W1566861922 cites W1983488837 @default.
- W1566861922 cites W1988561600 @default.
- W1566861922 cites W1989080074 @default.
- W1566861922 cites W1989651677 @default.
- W1566861922 cites W1998782381 @default.
- W1566861922 cites W2016049770 @default.
- W1566861922 cites W2026884660 @default.
- W1566861922 cites W2031937667 @default.
- W1566861922 cites W2049158685 @default.
- W1566861922 cites W2066204827 @default.
- W1566861922 cites W2077221259 @default.
- W1566861922 cites W2078961921 @default.
- W1566861922 cites W2086174182 @default.
- W1566861922 cites W2087234479 @default.
- W1566861922 cites W2091604172 @default.
- W1566861922 cites W2095162547 @default.
- W1566861922 cites W2095385679 @default.
- W1566861922 cites W2120054595 @default.
- W1566861922 cites W2138422562 @default.
- W1566861922 cites W2460698065 @default.
- W1566861922 cites W3004816780 @default.
- W1566861922 doi "https://doi.org/10.1016/s0021-9258(17)43326-6" @default.
- W1566861922 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/6321460" @default.
- W1566861922 hasPublicationYear "1984" @default.
- W1566861922 type Work @default.
- W1566861922 sameAs 1566861922 @default.
- W1566861922 citedByCount "21" @default.
- W1566861922 crossrefType "journal-article" @default.
- W1566861922 hasAuthorship W1566861922A5000872039 @default.
- W1566861922 hasAuthorship W1566861922A5020813055 @default.
- W1566861922 hasAuthorship W1566861922A5050920574 @default.
- W1566861922 hasBestOaLocation W15668619221 @default.
- W1566861922 hasConcept C104317684 @default.
- W1566861922 hasConcept C107824862 @default.
- W1566861922 hasConcept C185592680 @default.
- W1566861922 hasConcept C24107716 @default.
- W1566861922 hasConcept C51639874 @default.
- W1566861922 hasConcept C553089730 @default.
- W1566861922 hasConcept C55493867 @default.
- W1566861922 hasConcept C86339819 @default.
- W1566861922 hasConcept C94966510 @default.
- W1566861922 hasConceptScore W1566861922C104317684 @default.
- W1566861922 hasConceptScore W1566861922C107824862 @default.
- W1566861922 hasConceptScore W1566861922C185592680 @default.
- W1566861922 hasConceptScore W1566861922C24107716 @default.
- W1566861922 hasConceptScore W1566861922C51639874 @default.
- W1566861922 hasConceptScore W1566861922C553089730 @default.
- W1566861922 hasConceptScore W1566861922C55493867 @default.
- W1566861922 hasConceptScore W1566861922C86339819 @default.
- W1566861922 hasConceptScore W1566861922C94966510 @default.
- W1566861922 hasIssue "4" @default.
- W1566861922 hasLocation W15668619221 @default.
- W1566861922 hasOpenAccess W1566861922 @default.
- W1566861922 hasPrimaryLocation W15668619221 @default.
- W1566861922 hasRelatedWork W1598958185 @default.
- W1566861922 hasRelatedWork W1975021939 @default.
- W1566861922 hasRelatedWork W1990921346 @default.
- W1566861922 hasRelatedWork W2074463981 @default.
- W1566861922 hasRelatedWork W2077108160 @default.
- W1566861922 hasRelatedWork W2102845745 @default.
- W1566861922 hasRelatedWork W2122839549 @default.
- W1566861922 hasRelatedWork W2158818332 @default.
- W1566861922 hasRelatedWork W2314536821 @default.
- W1566861922 hasRelatedWork W2181394363 @default.
- W1566861922 hasVolume "259" @default.
- W1566861922 isParatext "false" @default.
- W1566861922 isRetracted "false" @default.
- W1566861922 magId "1566861922" @default.
- W1566861922 workType "article" @default.