Matches in SemOpenAlex for { <https://semopenalex.org/work/W1566984855> ?p ?o ?g. }
Showing items 1 to 72 of
72
with 100 items per page.
- W1566984855 endingPage "9" @default.
- W1566984855 startingPage "2973" @default.
- W1566984855 abstract "Previous work with rat and rabbit liver enzymes has demonstrated that cytochrome P450IIE1 is responsible for the metabolism of N-nitrosodimethylamine (NDMA), a widely occurring carcinogen. The present study demonstrated that a similar enzyme also exists in the mouse kidney and is regulated by testosterone. These results can account for the reported sex-related difference in the renal metabolism of NDMA in mouse strains such as C3H/HeJ. NDMA demethylase activities (expressed as pmol/min/mg protein) in kidney microsomes of female and male C3H/HeJ mice were 3.0 +/- 0.7 and 51.9 +/- 11.2, respectively. After testosterone treatment (500 mg/kg b.w. in olive oil, s.c.) for 2 days, the renal NDMA demethylase activity of the female mice was elevated 17-fold. The difference and change in NDMA demethylase activity were accompanied by corresponding differences and changes in P450IIE1 as quantified by immunoblot analysis (using antibodies prepared against rat P450IIE1) as well as in the mRNA level for P450IIE1 as determined by Northern and slot blot analyses (using a cDNA probe containing the coding sequence of rat P450IIE1 gene). Based on gel electrophoresis, the molecular weight of mouse renal P450IIE1 was 52,000 and the size of mouse renal P450IIE1 mRNA was approximately 1.8 kilobases; both were similar to those found in rat liver and kidney. Renal P450IIE1 mRNA levels in female, male, and testosterone-treated female mice were at a ratio of 1:22:20. On the other hand, this testosterone-related difference was not observed in hepatic P450IIE1. In liver microsomes, there were no significant differences in NDMA demethylase activity, P450IIE1 content, and P450IIE1 mRNA level between male and female mice or between untreated and testosterone-treated female mice. The apparent Km value of NDMA demethylase in mouse kidney microsomes (22 to 27 microM NDMA) were similar to that in rat liver microsomes. Renal NDMA demethylase activity was inhibited by a monoclonal antibody prepared against rat P450IIE1. These results suggest that mouse renal P450IIE1 is similar to rat P450IIE1 and is responsible for the low Km form of NDMA demethylase activity. Nevertheless, only the mouse renal enzyme is regulated by testosterone." @default.
- W1566984855 created "2016-06-24" @default.
- W1566984855 creator A5078652096 @default.
- W1566984855 creator A5079143963 @default.
- W1566984855 creator A5086183507 @default.
- W1566984855 creator A5086188593 @default.
- W1566984855 date "1989-06-01" @default.
- W1566984855 modified "2023-09-23" @default.
- W1566984855 title "Molecular basis for the sex-related difference in renal N-nitrosodimethylamine demethylase in C3H/HeJ mice." @default.
- W1566984855 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/2720658" @default.
- W1566984855 hasPublicationYear "1989" @default.
- W1566984855 type Work @default.
- W1566984855 sameAs 1566984855 @default.
- W1566984855 citedByCount "11" @default.
- W1566984855 crossrefType "journal-article" @default.
- W1566984855 hasAuthorship W1566984855A5078652096 @default.
- W1566984855 hasAuthorship W1566984855A5079143963 @default.
- W1566984855 hasAuthorship W1566984855A5086183507 @default.
- W1566984855 hasAuthorship W1566984855A5086188593 @default.
- W1566984855 hasConcept C104317684 @default.
- W1566984855 hasConcept C114246631 @default.
- W1566984855 hasConcept C126322002 @default.
- W1566984855 hasConcept C134018914 @default.
- W1566984855 hasConcept C181199279 @default.
- W1566984855 hasConcept C185592680 @default.
- W1566984855 hasConcept C2779279991 @default.
- W1566984855 hasConcept C2780091579 @default.
- W1566984855 hasConcept C2781364455 @default.
- W1566984855 hasConcept C41091548 @default.
- W1566984855 hasConcept C526171541 @default.
- W1566984855 hasConcept C55493867 @default.
- W1566984855 hasConcept C62231903 @default.
- W1566984855 hasConcept C71924100 @default.
- W1566984855 hasConcept C86803240 @default.
- W1566984855 hasConcept C87644729 @default.
- W1566984855 hasConceptScore W1566984855C104317684 @default.
- W1566984855 hasConceptScore W1566984855C114246631 @default.
- W1566984855 hasConceptScore W1566984855C126322002 @default.
- W1566984855 hasConceptScore W1566984855C134018914 @default.
- W1566984855 hasConceptScore W1566984855C181199279 @default.
- W1566984855 hasConceptScore W1566984855C185592680 @default.
- W1566984855 hasConceptScore W1566984855C2779279991 @default.
- W1566984855 hasConceptScore W1566984855C2780091579 @default.
- W1566984855 hasConceptScore W1566984855C2781364455 @default.
- W1566984855 hasConceptScore W1566984855C41091548 @default.
- W1566984855 hasConceptScore W1566984855C526171541 @default.
- W1566984855 hasConceptScore W1566984855C55493867 @default.
- W1566984855 hasConceptScore W1566984855C62231903 @default.
- W1566984855 hasConceptScore W1566984855C71924100 @default.
- W1566984855 hasConceptScore W1566984855C86803240 @default.
- W1566984855 hasConceptScore W1566984855C87644729 @default.
- W1566984855 hasIssue "11" @default.
- W1566984855 hasLocation W15669848551 @default.
- W1566984855 hasOpenAccess W1566984855 @default.
- W1566984855 hasPrimaryLocation W15669848551 @default.
- W1566984855 hasRelatedWork W1966561000 @default.
- W1566984855 hasRelatedWork W1980451061 @default.
- W1566984855 hasRelatedWork W1996780733 @default.
- W1566984855 hasRelatedWork W2001509668 @default.
- W1566984855 hasRelatedWork W2003997084 @default.
- W1566984855 hasRelatedWork W2007974967 @default.
- W1566984855 hasRelatedWork W2069381629 @default.
- W1566984855 hasRelatedWork W2108572185 @default.
- W1566984855 hasRelatedWork W2313856518 @default.
- W1566984855 hasRelatedWork W2008246658 @default.
- W1566984855 hasVolume "49" @default.
- W1566984855 isParatext "false" @default.
- W1566984855 isRetracted "false" @default.
- W1566984855 magId "1566984855" @default.
- W1566984855 workType "article" @default.