Matches in SemOpenAlex for { <https://semopenalex.org/work/W1567106587> ?p ?o ?g. }
Showing items 1 to 84 of
84
with 100 items per page.
- W1567106587 endingPage "436" @default.
- W1567106587 startingPage "433" @default.
- W1567106587 abstract "Abstract The quenching of liver alcohol dehydrogenase protein fluorescence at alkaline pH indicates two conformational states of the enzyme with a pKa of 9.8+/-0.2, shifted to 10.6+/-0.2 in D2O. NAD+ and 2-p-toluidinonaphthalene-6-sulfonate, a fluorescent probe competitive with coenzyme, bind to the acid conformation of the enzyme. The pKa of the protein-fluorescence quenching curve is shifted toward 7.6 in the presence of NAD+, and the ternary complex formation with NAD+ and trifluoroethanol results in a pH-independent maximal quench. At pH (pD) 10.5, the rate constant for NAD+ binding was 2.6 times faster in D2O2 than in H2O due to the shift of the pKa. Based on these results, a scheme has been proposed in which the state of protonation of an enzyme functional group with a pKa of 9.8 controls the conformational state of the enzyme. NAD+ binds to the acid conformation and subsequently causes another conformational change resulting in the perturbation of the pKa to 7.6. Alcohol then binds to the unprotonated form of the functional group with a pKa of 7.6 in the binary enzyme-NAD+ complex and converts the enzyme to the alkaline conformation. Thus, at neutral pH liver alcohol dehydrogenase undergoes two conformational changes en route to the ternary complex in which hydride transfer occurs." @default.
- W1567106587 created "2016-06-24" @default.
- W1567106587 creator A5009226007 @default.
- W1567106587 creator A5016958231 @default.
- W1567106587 creator A5039706629 @default.
- W1567106587 creator A5074085891 @default.
- W1567106587 date "1977-01-01" @default.
- W1567106587 modified "2023-10-13" @default.
- W1567106587 title "pH-dependent conformational states of horse liver alcohol dehydrogenase." @default.
- W1567106587 cites W120446455 @default.
- W1567106587 cites W1483206164 @default.
- W1567106587 cites W1533946522 @default.
- W1567106587 cites W1885778179 @default.
- W1567106587 cites W1911139910 @default.
- W1567106587 cites W1984174207 @default.
- W1567106587 cites W1987941631 @default.
- W1567106587 cites W2021603158 @default.
- W1567106587 cites W2036735604 @default.
- W1567106587 cites W2043392162 @default.
- W1567106587 cites W2053613947 @default.
- W1567106587 cites W2055550103 @default.
- W1567106587 cites W2057832053 @default.
- W1567106587 cites W2058236594 @default.
- W1567106587 cites W2060837765 @default.
- W1567106587 cites W2063802505 @default.
- W1567106587 cites W2067398719 @default.
- W1567106587 cites W2074930480 @default.
- W1567106587 cites W2084302847 @default.
- W1567106587 cites W2232248323 @default.
- W1567106587 cites W2329174981 @default.
- W1567106587 doi "https://doi.org/10.1016/s0021-9258(17)32734-5" @default.
- W1567106587 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/13068" @default.
- W1567106587 hasPublicationYear "1977" @default.
- W1567106587 type Work @default.
- W1567106587 sameAs 1567106587 @default.
- W1567106587 citedByCount "44" @default.
- W1567106587 countsByYear W15671065872023 @default.
- W1567106587 crossrefType "journal-article" @default.
- W1567106587 hasAuthorship W1567106587A5009226007 @default.
- W1567106587 hasAuthorship W1567106587A5016958231 @default.
- W1567106587 hasAuthorship W1567106587A5039706629 @default.
- W1567106587 hasAuthorship W1567106587A5074085891 @default.
- W1567106587 hasBestOaLocation W15671065871 @default.
- W1567106587 hasConcept C151730666 @default.
- W1567106587 hasConcept C181199279 @default.
- W1567106587 hasConcept C185592680 @default.
- W1567106587 hasConcept C2777344606 @default.
- W1567106587 hasConcept C2780762169 @default.
- W1567106587 hasConcept C2781066024 @default.
- W1567106587 hasConcept C2781415988 @default.
- W1567106587 hasConcept C55493867 @default.
- W1567106587 hasConcept C75520062 @default.
- W1567106587 hasConcept C86803240 @default.
- W1567106587 hasConceptScore W1567106587C151730666 @default.
- W1567106587 hasConceptScore W1567106587C181199279 @default.
- W1567106587 hasConceptScore W1567106587C185592680 @default.
- W1567106587 hasConceptScore W1567106587C2777344606 @default.
- W1567106587 hasConceptScore W1567106587C2780762169 @default.
- W1567106587 hasConceptScore W1567106587C2781066024 @default.
- W1567106587 hasConceptScore W1567106587C2781415988 @default.
- W1567106587 hasConceptScore W1567106587C55493867 @default.
- W1567106587 hasConceptScore W1567106587C75520062 @default.
- W1567106587 hasConceptScore W1567106587C86803240 @default.
- W1567106587 hasIssue "2" @default.
- W1567106587 hasLocation W15671065871 @default.
- W1567106587 hasOpenAccess W1567106587 @default.
- W1567106587 hasPrimaryLocation W15671065871 @default.
- W1567106587 hasRelatedWork W1504377868 @default.
- W1567106587 hasRelatedWork W2055574756 @default.
- W1567106587 hasRelatedWork W2074052481 @default.
- W1567106587 hasRelatedWork W2080807204 @default.
- W1567106587 hasRelatedWork W2147170700 @default.
- W1567106587 hasRelatedWork W2149157471 @default.
- W1567106587 hasRelatedWork W2160122090 @default.
- W1567106587 hasRelatedWork W2412724792 @default.
- W1567106587 hasRelatedWork W2606908362 @default.
- W1567106587 hasRelatedWork W2916382420 @default.
- W1567106587 hasVolume "252" @default.
- W1567106587 isParatext "false" @default.
- W1567106587 isRetracted "false" @default.
- W1567106587 magId "1567106587" @default.
- W1567106587 workType "article" @default.