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- W1567156813 abstract "This chapter describes the preparation of a specific adsorbent for thymidylate synthetase involving attachment of the substrate, 2'-deoxyuridylic acid (dUrd-5'-P) to agarose via an, ω-aminoalkyl “arm.” This material has been used successfully to purify the enzyme from a crude extract of lysed Lactobacillus casei cells. The partial purification of thymidylate synthetase from a number of bacterial and animal sources has been achieved by multistep sequences of conventional procedures. Recently Crusberg and Dunlap have reported the complete purification of thymidylate synthetase from antifolate-resistant strains of Lactobacillus casei. However, the purification of the mammalian enzyme has been hampered by the labile nature of this protein and low enzyme levels in cells and tissues. This disadvantage is especially evident in the case of Ehrlich ascites carcinoma cells and if reasonable quantities of enzyme are required for study, the usual procedures employed in purification become quite laborious. It is apparent that a single-step procedure based on affinity chromatography should greatly facilitate the purification process as well as enhance the recovery of enzyme activity." @default.
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- W1567156813 date "1974-01-01" @default.
- W1567156813 modified "2023-09-27" @default.
- W1567156813 title "[64] Purification of thymidylate synthetase with 2′-deoxyuridylate-agarose" @default.
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- W1567156813 doi "https://doi.org/10.1016/s0076-6879(74)34067-0" @default.
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