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• W1567166697 endingPage "6667" @default.
• W1567166697 startingPage "6656" @default.
• W1567166697 abstract "The surfaces of three Bacillus thuringiensis strains possess an S layer composed of linear arrays of small particles arranged with p2 symmetry and with a = 8.5 nm, b = 7.2 nm, and gamma = 73 degrees. Platinum shadows of whole cells and S-layer fragments revealed the outer surface of the array to be smooth and the inner surface to be corrugated. Treatment with 2 M guanidine hydrochloride at pH 2.5 to 4 best removed the S layer for chemical characterization; it was a relatively hydrophilic 91.4-kilodalton protein with a pI of 5, no detectable carbohydrate, cysteine, methionine or tryptophan, and 21.2% nonpolar residues. No N-terminal homology with other S-layer proteins was evident. Antibody labeling experiments confirmed that the amount of S layer was proportional to the growth phase in broth cultures. Late-exponential- and stationary-growth-phase cells typically sloughed off fragments of S layer, and this may be the result of wall turnover. Indigenous autolytic activity in isolated walls rapidly digested the wall fabric, liberating soluble S-layer protein. At the same time, proteases frequently reduced the molecular weight of the 91.4-kilodalton protein, but these polypeptides could still be identified as S-layer components by immunoblotting. As cultures were serially subcultured, the frequency of appearance of the S layer diminished, and it was eventually lost. The dynamic nature of this S layer makes it atypical of most previously identified S layers and made it unusually difficult to characterize." @default.
• W1567166697 created "2016-06-24" @default.
• W1567166697 creator A5045089465 @default.
• W1567166697 creator A5060092452 @default.
• W1567166697 date "1989-12-01" @default.
• W1567166697 modified "2023-10-11" @default.
• W1567166697 title "Characterization of a dynamic S layer on Bacillus thuringiensis" @default.
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• W1567166697 doi "https://doi.org/10.1128/jb.171.12.6656-6667.1989" @default.
• W1567166697 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/210560" @default.
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