Matches in SemOpenAlex for { <https://semopenalex.org/work/W1567169992> ?p ?o ?g. }
Showing items 1 to 65 of
65
with 100 items per page.
- W1567169992 abstract "ABSTRACTAdult bone marrow mesenchymal stem cells have the ability to differentiate into different cell types such as osteoblasts, adipocytes, chondrocytes, myoblasts, neural cells etc. The multipotency of mesenchymal stem cells, its easy isolation and culture as wells as their high ex vivo expansion potential make these cells to be exploited for therapeutic purpose. Despite enormous data available at molecular and cellular levels, it is evident that number of fundamental queries regarding molecular and cellular mechanisms needs to be elucidated. The present study is aimed at understanding differentiation of osteoblasts from mesenchymal stem cells (MSC) by certain candidate genes. shRNA based library screening was performed in our laboratory to silence specific mRNA that interfere with osteoblasts differentiation from MSC. shRNA library led to the identification of several candidate genes of known as well as unknown function. We focused our studies on candidate genes whose function is unknown and termed as osteoblasts inducer (ObI/OB). Current study is limited to understand a particular gene termed as ‘OB3’ in succession to our previously identified and characterized genes. OB3 gene identified during shRNA library screening was validated in both bone marrow stromal cell lines as well as murine primary mesenchymal stem cells. Its expression during osteoblasts differentiation has to been elucidated and its silencing effects on bone markers has been shown. We have by microarray few targets that are repressed upon OB3 silencing and involved in osteoblasts differentiation. Moreover, OB3 protein undergoes proteasome degradation and we have attempted if not completely to show that OB3 degradation is mediated through ubiquitin-proteasome.We have identified Creb3 as a partner of OB3 and have attempted to show creb3 silencing during osteoblasts differentiation. Furthermore overexpression of both OB3 and Creb3 independently delay osteoblasts differentiation. Attempts to identify the molecular mechanisms through which these two candidate genes are involved in osteoblasts differentiation is being explored." @default.
- W1567169992 created "2016-06-24" @default.
- W1567169992 creator A5073689671 @default.
- W1567169992 date "2012-02-01" @default.
- W1567169992 modified "2023-09-27" @default.
- W1567169992 title "Identification of the role of a novel conserved leucine Zipper – containing protein in osteogenic differentiation of mesenchymal stem cells" @default.
- W1567169992 hasPublicationYear "2012" @default.
- W1567169992 type Work @default.
- W1567169992 sameAs 1567169992 @default.
- W1567169992 citedByCount "0" @default.
- W1567169992 crossrefType "journal-article" @default.
- W1567169992 hasAuthorship W1567169992A5073689671 @default.
- W1567169992 hasConcept C104317684 @default.
- W1567169992 hasConcept C148738053 @default.
- W1567169992 hasConcept C173396325 @default.
- W1567169992 hasConcept C190232843 @default.
- W1567169992 hasConcept C198826908 @default.
- W1567169992 hasConcept C202751555 @default.
- W1567169992 hasConcept C2776363554 @default.
- W1567169992 hasConcept C2778260815 @default.
- W1567169992 hasConcept C28328180 @default.
- W1567169992 hasConcept C54355233 @default.
- W1567169992 hasConcept C81885089 @default.
- W1567169992 hasConcept C86803240 @default.
- W1567169992 hasConcept C95444343 @default.
- W1567169992 hasConceptScore W1567169992C104317684 @default.
- W1567169992 hasConceptScore W1567169992C148738053 @default.
- W1567169992 hasConceptScore W1567169992C173396325 @default.
- W1567169992 hasConceptScore W1567169992C190232843 @default.
- W1567169992 hasConceptScore W1567169992C198826908 @default.
- W1567169992 hasConceptScore W1567169992C202751555 @default.
- W1567169992 hasConceptScore W1567169992C2776363554 @default.
- W1567169992 hasConceptScore W1567169992C2778260815 @default.
- W1567169992 hasConceptScore W1567169992C28328180 @default.
- W1567169992 hasConceptScore W1567169992C54355233 @default.
- W1567169992 hasConceptScore W1567169992C81885089 @default.
- W1567169992 hasConceptScore W1567169992C86803240 @default.
- W1567169992 hasConceptScore W1567169992C95444343 @default.
- W1567169992 hasLocation W15671699921 @default.
- W1567169992 hasOpenAccess W1567169992 @default.
- W1567169992 hasPrimaryLocation W15671699921 @default.
- W1567169992 hasRelatedWork W1496926840 @default.
- W1567169992 hasRelatedWork W1961169397 @default.
- W1567169992 hasRelatedWork W1979121282 @default.
- W1567169992 hasRelatedWork W2004747651 @default.
- W1567169992 hasRelatedWork W2021227930 @default.
- W1567169992 hasRelatedWork W2069758652 @default.
- W1567169992 hasRelatedWork W2071249835 @default.
- W1567169992 hasRelatedWork W2073516028 @default.
- W1567169992 hasRelatedWork W2075291842 @default.
- W1567169992 hasRelatedWork W2079187063 @default.
- W1567169992 hasRelatedWork W2081605424 @default.
- W1567169992 hasRelatedWork W2126263118 @default.
- W1567169992 hasRelatedWork W2283620016 @default.
- W1567169992 hasRelatedWork W2462937711 @default.
- W1567169992 hasRelatedWork W2587406494 @default.
- W1567169992 hasRelatedWork W2967401795 @default.
- W1567169992 hasRelatedWork W2967878857 @default.
- W1567169992 hasRelatedWork W3134718549 @default.
- W1567169992 hasRelatedWork W3195508794 @default.
- W1567169992 hasRelatedWork W840868764 @default.
- W1567169992 isParatext "false" @default.
- W1567169992 isRetracted "false" @default.
- W1567169992 magId "1567169992" @default.
- W1567169992 workType "article" @default.