FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1567182159> ?p ?o ?g. }

• W1567182159 endingPage "10628" @default.
• W1567182159 startingPage "10621" @default.
• W1567182159 abstract "Rat hepatocytes have been studied in suspension culture for 10-h periods. Levels of extractable lactate dehydrogenase (LDH) have been measured in these hepatocytes at hourly intervals in order to note the balance between biosynthesis and degradation of this enzyme. Newly synthesized LDH has been measured by following the rate of incorporation of [3H]leucine into radiochemically pure LDH of high specific catalytic activity as isolated by a rapid affinity chromatographic procedure. The effects of the addition of physiological concentrations of the following hormones at the beginning of 10-h culture periods immediately following preparation of the hepatocytes by the collagen perfusion procedure have been recorded. The hormones triiodothyronine (T3), insulin, glucagon, and dexamethasone have been added singly or in combination. The culture medium has supplied variable amounts of these hormones in the 10% of fetal calf (or other) serum added, and the hepatocytes themselves have provided intracellular amounts of hormones. In addition to the added hormones, N6,O2'-dibutyryl cyclic AMP (Bt2cAMP) has also been studied. Control suspensions of hepatocytes show reproducible initial levels of extractable LDH which are maintained or slightly increased during 10 h. Such control systems also incorporate [3H]leucine into total protein and into highly purified LDH at reproducible rates during 10 h of incubation. The effects of added hormones on LDH lavels are as follows: (a) T3 causes about a 2-fold increase in LDH at 7 to 8 h in hepatocytes from young adult animals, an effect which is lowered in either younger or older animals or in thyroidectomized animals. (b) Insulin leads to a similar increase in LDH at 5 to 6 h and a falling off at 8 to 10 h. (c) Glucagon also causes an approximate doubling of the amount of extractable LDH during a 10-doubling of the amount of extractable LDH during a 10-h period. (d) Dexamethasone does not produce an increase. (e) Bt2-cAMP produces an effect indistinguishable from that of glucagon. Paired combinations of these hormones fail to produce an additive response in any case. The combinations of T3 plus dexamethaseon and insulin plus dexamethasone lead to significant reductions in levels of extractable LDH when compared to the single hormone effects cited above. With respect to rates of synthesis of total protein as measured by [3H]leucine incorporation, only glucagon, glucagon plus Bt2-cAMP, glucagon plus insulin, T3 plus Bt2cAMP, and T3 plus insulin produce significant increases during a 10-h period. However, when [3H]leucine incorporation into highly purified LDH is measured as an index of LDH biosynthesis, T3, insulin, and glucagon consistently increase the biosynthetic rates during a 10-h period. Bt2cAMP produces a smaller increase. Dexamethasone fails to produce any significant change when compared to controls. Paired combinations of hormones again do not produce any additive effect on LDH biosynthesis when the hormone producing the higher level is taken as the reference..." @default.
• W1567182159 created "2016-06-24" @default.
• W1567182159 creator A5027717667 @default.
• W1567182159 creator A5080506555 @default.
• W1567182159 date "1979-11-01" @default.
• W1567182159 modified "2023-09-28" @default.
• W1567182159 title "Hormonal effects on the biosynthesis of lactate dehydrogenase in rat hepatocytes." @default.
• W1567182159 cites W1029517436 @default.
• W1567182159 cites W1496379136 @default.
• W1567182159 cites W1498996659 @default.
• W1567182159 cites W1509698999 @default.
• W1567182159 cites W1515245060 @default.
• W1567182159 cites W1519449747 @default.
• W1567182159 cites W1541647579 @default.
• W1567182159 cites W1542333814 @default.
• W1567182159 cites W1547202268 @default.
• W1567182159 cites W1548659851 @default.
• W1567182159 cites W1549027440 @default.
• W1567182159 cites W1568700466 @default.
• W1567182159 cites W158663427 @default.
• W1567182159 cites W1596570248 @default.
• W1567182159 cites W1604887498 @default.
• W1567182159 cites W1667518818 @default.
• W1567182159 cites W167767438 @default.
• W1567182159 cites W1775749144 @default.
• W1567182159 cites W1972223728 @default.
• W1567182159 cites W1976039913 @default.
• W1567182159 cites W1979933243 @default.
• W1567182159 cites W1987375895 @default.
• W1567182159 cites W1994182953 @default.
• W1567182159 cites W2000815636 @default.
• W1567182159 cites W2006391324 @default.
• W1567182159 cites W2012966827 @default.
• W1567182159 cites W2014579000 @default.
• W1567182159 cites W2023229316 @default.
• W1567182159 cites W2023857291 @default.
• W1567182159 cites W2031479527 @default.
• W1567182159 cites W2040716895 @default.
• W1567182159 cites W2062012459 @default.
• W1567182159 cites W2064952052 @default.
• W1567182159 cites W2066273026 @default.
• W1567182159 cites W2070543423 @default.
• W1567182159 cites W2072032071 @default.
• W1567182159 cites W2072482644 @default.
• W1567182159 cites W2074877984 @default.
• W1567182159 cites W2077450713 @default.
• W1567182159 cites W2077844789 @default.
• W1567182159 cites W2091631776 @default.
• W1567182159 cites W2094715960 @default.
• W1567182159 cites W2108358343 @default.
• W1567182159 cites W2109401564 @default.
• W1567182159 cites W2123917437 @default.
• W1567182159 cites W2129307251 @default.
• W1567182159 cites W2143406927 @default.
• W1567182159 cites W2148076576 @default.
• W1567182159 cites W2164226796 @default.
• W1567182159 cites W2204008620 @default.
• W1567182159 cites W2335713330 @default.
• W1567182159 cites W2342491556 @default.
• W1567182159 cites W2395339465 @default.
• W1567182159 cites W2397048455 @default.
• W1567182159 cites W2409499000 @default.
• W1567182159 cites W2410475055 @default.
• W1567182159 cites W2410488314 @default.
• W1567182159 cites W2416664380 @default.
• W1567182159 cites W2419377870 @default.
• W1567182159 cites W2430332946 @default.
• W1567182159 cites W70760971 @default.
• W1567182159 doi "https://doi.org/10.1016/s0021-9258(19)86565-1" @default.
• W1567182159 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/227847" @default.
• W1567182159 hasPublicationYear "1979" @default.
• W1567182159 type Work @default.
• W1567182159 sameAs 1567182159 @default.
• W1567182159 citedByCount "13" @default.
• W1567182159 countsByYear W15671821592014 @default.
• W1567182159 crossrefType "journal-article" @default.
• W1567182159 hasAuthorship W1567182159A5027717667 @default.
• W1567182159 hasAuthorship W1567182159A5080506555 @default.
• W1567182159 hasBestOaLocation W15671821591 @default.
• W1567182159 hasConcept C181199279 @default.
• W1567182159 hasConcept C185592680 @default.
• W1567182159 hasConcept C2776317432 @default.
• W1567182159 hasConcept C2777318727 @default.
• W1567182159 hasConcept C2909544887 @default.
• W1567182159 hasConcept C553450214 @default.
• W1567182159 hasConcept C55493867 @default.
• W1567182159 hasConcept C71315377 @default.
• W1567182159 hasConcept C86803240 @default.
• W1567182159 hasConceptScore W1567182159C181199279 @default.
• W1567182159 hasConceptScore W1567182159C185592680 @default.
• W1567182159 hasConceptScore W1567182159C2776317432 @default.
• W1567182159 hasConceptScore W1567182159C2777318727 @default.
• W1567182159 hasConceptScore W1567182159C2909544887 @default.
• W1567182159 hasConceptScore W1567182159C553450214 @default.
• W1567182159 hasConceptScore W1567182159C55493867 @default.
• W1567182159 hasConceptScore W1567182159C71315377 @default.
• W1567182159 hasConceptScore W1567182159C86803240 @default.
• W1567182159 hasIssue "21" @default.

• next