Matches in SemOpenAlex for { <https://semopenalex.org/work/W1567185751> ?p ?o ?g. }
Showing items 1 to 92 of
92
with 100 items per page.
- W1567185751 endingPage "1805" @default.
- W1567185751 startingPage "1800" @default.
- W1567185751 abstract "The reactivity of recombinant and tumor-derived preparations of oncomodulin toward 5,5'-dithiobis-(2-nitrobenzoate) (Ellman's reagent) and dansylaziridine was investigated. In contrast to previously published data (Mutus, B., Palmer, E. J., and MacManus, J. P. (1988) Biochemistry 27, 5615-5622), the apoprotein was observed to react far more rapidly than the calcium-bound form with Ellman's reagent. Attempts to quantitatively label the native protein with dansylaziridine met with little success, either with the metal-free or calcium-bound forms. In neither case did the extent of modification approach the level observed with the sodium dodecyl sulfate-denatured form of the protein. These results suggest that access to the sulfhydryl group of Cys-18 is severely restricted in the native protein, particularly when the high affinity ion-binding sites are occupied. Consistent with these observations, prolonged incubation of native oncomodulin at room temperature in the absence of reductant did not result in the generation of disulfide-linked dimers, either in the presence or absence of Ca2+. Interestingly, however, Cu2+ ion was observed to facilitate the apparent dimerization of oncomodulin. This reaction, which occurs more rapidly with the Ca2(+)-free form of the protein, affords material with the expected electrophoretic mobility. However, in contrast with the results of Mutus et al., dimeric oncomodulin prepared in this manner fails to stimulate bovine heart cAMP phosphodiesterase." @default.
- W1567185751 created "2016-06-24" @default.
- W1567185751 creator A5005785760 @default.
- W1567185751 creator A5041179608 @default.
- W1567185751 creator A5049941212 @default.
- W1567185751 date "1990-01-01" @default.
- W1567185751 modified "2023-09-28" @default.
- W1567185751 title "Reactivity of cysteine 18 in oncomodulin." @default.
- W1567185751 cites W1503782530 @default.
- W1567185751 cites W1509910850 @default.
- W1567185751 cites W1541324220 @default.
- W1567185751 cites W1546969865 @default.
- W1567185751 cites W1555801234 @default.
- W1567185751 cites W1584385555 @default.
- W1567185751 cites W1596012657 @default.
- W1567185751 cites W189687668 @default.
- W1567185751 cites W1957414838 @default.
- W1567185751 cites W1985367264 @default.
- W1567185751 cites W1993076506 @default.
- W1567185751 cites W1998296250 @default.
- W1567185751 cites W1998356438 @default.
- W1567185751 cites W2006370393 @default.
- W1567185751 cites W2006799708 @default.
- W1567185751 cites W2014920358 @default.
- W1567185751 cites W2017321765 @default.
- W1567185751 cites W2025990187 @default.
- W1567185751 cites W2029214893 @default.
- W1567185751 cites W2029625486 @default.
- W1567185751 cites W2039366113 @default.
- W1567185751 cites W2040711699 @default.
- W1567185751 cites W2057491726 @default.
- W1567185751 cites W2058359928 @default.
- W1567185751 cites W2064057205 @default.
- W1567185751 cites W2071454945 @default.
- W1567185751 cites W2074745266 @default.
- W1567185751 cites W2094324563 @default.
- W1567185751 cites W2119460480 @default.
- W1567185751 cites W2134217220 @default.
- W1567185751 cites W2204636683 @default.
- W1567185751 cites W4211204946 @default.
- W1567185751 doi "https://doi.org/10.1016/s0021-9258(19)40088-4" @default.
- W1567185751 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/2153144" @default.
- W1567185751 hasPublicationYear "1990" @default.
- W1567185751 type Work @default.
- W1567185751 sameAs 1567185751 @default.
- W1567185751 citedByCount "20" @default.
- W1567185751 countsByYear W15671857512018 @default.
- W1567185751 countsByYear W15671857512019 @default.
- W1567185751 countsByYear W15671857512021 @default.
- W1567185751 countsByYear W15671857512022 @default.
- W1567185751 crossrefType "journal-article" @default.
- W1567185751 hasAuthorship W1567185751A5005785760 @default.
- W1567185751 hasAuthorship W1567185751A5041179608 @default.
- W1567185751 hasAuthorship W1567185751A5049941212 @default.
- W1567185751 hasBestOaLocation W15671857511 @default.
- W1567185751 hasConcept C142724271 @default.
- W1567185751 hasConcept C181199279 @default.
- W1567185751 hasConcept C185592680 @default.
- W1567185751 hasConcept C204787440 @default.
- W1567185751 hasConcept C2776910235 @default.
- W1567185751 hasConcept C2779201268 @default.
- W1567185751 hasConcept C55493867 @default.
- W1567185751 hasConcept C71924100 @default.
- W1567185751 hasConceptScore W1567185751C142724271 @default.
- W1567185751 hasConceptScore W1567185751C181199279 @default.
- W1567185751 hasConceptScore W1567185751C185592680 @default.
- W1567185751 hasConceptScore W1567185751C204787440 @default.
- W1567185751 hasConceptScore W1567185751C2776910235 @default.
- W1567185751 hasConceptScore W1567185751C2779201268 @default.
- W1567185751 hasConceptScore W1567185751C55493867 @default.
- W1567185751 hasConceptScore W1567185751C71924100 @default.
- W1567185751 hasIssue "3" @default.
- W1567185751 hasLocation W15671857511 @default.
- W1567185751 hasOpenAccess W1567185751 @default.
- W1567185751 hasPrimaryLocation W15671857511 @default.
- W1567185751 hasRelatedWork W1976253167 @default.
- W1567185751 hasRelatedWork W2009996801 @default.
- W1567185751 hasRelatedWork W2397218192 @default.
- W1567185751 hasRelatedWork W2399033717 @default.
- W1567185751 hasRelatedWork W2402690918 @default.
- W1567185751 hasRelatedWork W2801688792 @default.
- W1567185751 hasRelatedWork W2949852058 @default.
- W1567185751 hasRelatedWork W2950013228 @default.
- W1567185751 hasRelatedWork W2951808849 @default.
- W1567185751 hasRelatedWork W2952991618 @default.
- W1567185751 hasVolume "265" @default.
- W1567185751 isParatext "false" @default.
- W1567185751 isRetracted "false" @default.
- W1567185751 magId "1567185751" @default.
- W1567185751 workType "article" @default.