Matches in SemOpenAlex for { <https://semopenalex.org/work/W1567199036> ?p ?o ?g. }
- W1567199036 endingPage "e0131868" @default.
- W1567199036 startingPage "e0131868" @default.
- W1567199036 abstract "Matrix proteins play important roles in shell formation. Our group firstly isolated three cDNAs encoding lysine-rich matrix protein from Pinctada fucata in 2006. However, the functions of KRMPs are not fully understood. In addition, KRMPs contain two functional domains, the basic domain and the Gly/Tyr domain respectively. Based on the modular organization, the roles of their two domains were poorly characterized. Furthermore, KRMPs were then reported in other two species, P. maxima and P. margaritifera, which indicated that KRMPs might be very important for shell formation. In this study, the characterization and function of KRMP-3 and its two functional domains were studied in vitro through purification of recombinant glutathione S-transferase tagged KRMP-3 and two KRMP-3 deletion mutants. Western blot and immunofluorescence revealed that native KRMP-3 existed in the EDTA-insoluble matrix of the prismatic layer and was located in the organic sheet and the prismatic sheath. Recombinant KRMP-3 (rKRMP-3) bound tightly to chitin and this binding capacity was duo to the Gly/Tyr-rich region. rKRMP-3 inhibited the precipitation of CaCO3, affected the crystal morphology of calcite and inhibited the growth of aragonite in vitro, which was almost entirely attributed to the lysine-rich region. The results present direct evidence of the roles of KRMP-3 in shell biomineralization. The functional rBR region was found to participate in the growth control of crystals and the rGYR region was responsible to bind to chitin." @default.
- W1567199036 created "2016-06-24" @default.
- W1567199036 creator A5001375054 @default.
- W1567199036 creator A5021472040 @default.
- W1567199036 creator A5026948419 @default.
- W1567199036 creator A5030142013 @default.
- W1567199036 creator A5046860067 @default.
- W1567199036 creator A5060955767 @default.
- W1567199036 creator A5067178534 @default.
- W1567199036 creator A5073726678 @default.
- W1567199036 creator A5086128236 @default.
- W1567199036 date "2015-07-10" @default.
- W1567199036 modified "2023-09-24" @default.
- W1567199036 title "Dual Roles of the Lysine-Rich Matrix Protein (KRMP)-3 in Shell Formation of Pearl Oyster, Pinctada fucata" @default.
- W1567199036 cites W1506364108 @default.
- W1567199036 cites W1521233004 @default.
- W1567199036 cites W1665727945 @default.
- W1567199036 cites W1970286129 @default.
- W1567199036 cites W1974671676 @default.
- W1567199036 cites W1974705035 @default.
- W1567199036 cites W1975049967 @default.
- W1567199036 cites W1977665189 @default.
- W1567199036 cites W1986598651 @default.
- W1567199036 cites W1989025984 @default.
- W1567199036 cites W1996497488 @default.
- W1567199036 cites W1997817450 @default.
- W1567199036 cites W2019427380 @default.
- W1567199036 cites W2019867710 @default.
- W1567199036 cites W2021032815 @default.
- W1567199036 cites W2022907134 @default.
- W1567199036 cites W2023372862 @default.
- W1567199036 cites W2024350113 @default.
- W1567199036 cites W2025809590 @default.
- W1567199036 cites W2029805969 @default.
- W1567199036 cites W2053747088 @default.
- W1567199036 cites W2071989281 @default.
- W1567199036 cites W2072611679 @default.
- W1567199036 cites W2078658097 @default.
- W1567199036 cites W2082695646 @default.
- W1567199036 cites W2082923993 @default.
- W1567199036 cites W2091871958 @default.
- W1567199036 cites W2095199125 @default.
- W1567199036 cites W2104717117 @default.
- W1567199036 cites W2118267499 @default.
- W1567199036 cites W2129129481 @default.
- W1567199036 cites W2137595306 @default.
- W1567199036 cites W2141398064 @default.
- W1567199036 cites W2144837992 @default.
- W1567199036 cites W2151526017 @default.
- W1567199036 cites W2158480602 @default.
- W1567199036 cites W2165388124 @default.
- W1567199036 cites W2166283140 @default.
- W1567199036 cites W2166413478 @default.
- W1567199036 cites W2166460930 @default.
- W1567199036 cites W2168744571 @default.
- W1567199036 cites W4230292448 @default.
- W1567199036 doi "https://doi.org/10.1371/journal.pone.0131868" @default.
- W1567199036 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4498902" @default.
- W1567199036 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/26161976" @default.
- W1567199036 hasPublicationYear "2015" @default.
- W1567199036 type Work @default.
- W1567199036 sameAs 1567199036 @default.
- W1567199036 citedByCount "28" @default.
- W1567199036 countsByYear W15671990362015 @default.
- W1567199036 countsByYear W15671990362016 @default.
- W1567199036 countsByYear W15671990362017 @default.
- W1567199036 countsByYear W15671990362018 @default.
- W1567199036 countsByYear W15671990362019 @default.
- W1567199036 countsByYear W15671990362020 @default.
- W1567199036 countsByYear W15671990362021 @default.
- W1567199036 countsByYear W15671990362022 @default.
- W1567199036 countsByYear W15671990362023 @default.
- W1567199036 crossrefType "journal-article" @default.
- W1567199036 hasAuthorship W1567199036A5001375054 @default.
- W1567199036 hasAuthorship W1567199036A5021472040 @default.
- W1567199036 hasAuthorship W1567199036A5026948419 @default.
- W1567199036 hasAuthorship W1567199036A5030142013 @default.
- W1567199036 hasAuthorship W1567199036A5046860067 @default.
- W1567199036 hasAuthorship W1567199036A5060955767 @default.
- W1567199036 hasAuthorship W1567199036A5067178534 @default.
- W1567199036 hasAuthorship W1567199036A5073726678 @default.
- W1567199036 hasAuthorship W1567199036A5086128236 @default.
- W1567199036 hasBestOaLocation W15671990361 @default.
- W1567199036 hasConcept C104317684 @default.
- W1567199036 hasConcept C12554922 @default.
- W1567199036 hasConcept C138885662 @default.
- W1567199036 hasConcept C151730666 @default.
- W1567199036 hasConcept C153911025 @default.
- W1567199036 hasConcept C183586093 @default.
- W1567199036 hasConcept C185592680 @default.
- W1567199036 hasConcept C27206212 @default.
- W1567199036 hasConcept C2776016237 @default.
- W1567199036 hasConcept C2776415932 @default.
- W1567199036 hasConcept C2779251273 @default.
- W1567199036 hasConcept C2779732960 @default.
- W1567199036 hasConcept C2779866092 @default.
- W1567199036 hasConcept C2780191791 @default.
- W1567199036 hasConcept C2781088250 @default.