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• W1567204298 abstract "Acridine orange, a fluorescent dye, has no measurable effect on cell multiplication, protein synthesis or DNA replication in L-strain fibroblasts in culture when shielded from light, in concentrations up to 5 × 10−7M (150 micrograms/100 ml). Acridine orange is readily demonstrable within nuclei at any time during the period of cell culture. Interphase and mitotic nuclei are equally well stained. Larger doses are toxic or lethal. The same effect is noted in cultures of Ehrlich ascites tumor cells and bacteria. Acridine orange is a photodynamic compound, and exposure of the cultures to light causes prompt cessation of growth of the culture, net protein synthesis and deoxyribonucleic acid replication. Cytochrome oxidase activity is insignificantly depressed in cells which have been damaged by irradiation of the photosensitized cells. The degree of depression is negligible as compared with cells grown in the virtual absence of oxygen and with Ehrlich ascites tumor cells. There is no degradation of acridine orange by L-cells in culture over a 4 day period, but there is some metabolic control over the dye, evidenced by the changing pattern of intracellular acridine orange content during the growth cycle of the culture." @default.
• W1567204298 created "2016-06-24" @default.
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• W1567204298 date "1960-10-01" @default.
• W1567204298 modified "2023-09-24" @default.
• W1567204298 title "Photosensitization of nucleic acids and proteins" @default.
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• W1567204298 doi "https://doi.org/10.1016/0014-4827(60)90351-7" @default.
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