FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1567227084> ?p ?o ?g. }

• W1567227084 endingPage "1850" @default.
• W1567227084 startingPage "1844" @default.
• W1567227084 abstract "The extracellular endo-1,4-beta-glucanase components of Ruminococcus flavefaciens FD-1 were analyzed by high-performance liquid chromatography (HPLC) by using DEAE ion-exchange, hydroxylapatite, and gel filtration chromatography and polyacrylamide gel electrophoresis (PAGE). Two endo-1,4-beta-glucanase peaks were resolved by DEAE-HPLC and termed endoglucanases A and B. Carboxymethyl cellulose (CMC) zymograms were achieved by enzyme separation using nondenaturing PAGE followed by incubation of the gel on top of a CMC-agarose gel. This revealed no less than 13 and 5 endo-1,4-beta-glucanase components present in endoglucanases A and B, respectively. Hydroxylapatite chromatography of endoglucanases A and B revealed one activity peak for each preparation, which contained 4 and 5 endo-1,4-beta-glucanase components, respectively. Gel filtration chromatography of endoglucanase A following hydroxylapatite chromatography resolved the most active carboxymethylcellulase (CMCase) component from other endo-1,4-beta-glucanase activities. Gel filtration of endoglucanase B following hydroxylapatite chromatography showed one CMCase activity peak. Protein stains of sodium dodecyl sulfate-PAGE and nondenaturing PAGE gels of endoglucanases A and B from hydroxylapatite and gel filtration chromatography revealed multiple protein components. When xylan was substituted for CMC in zymograms, identical separation patterns for CMCase and xylanase activities were observed for both endoglucanases A and B. These data suggest that both 1,4-beta linkage-hydrolyzing activities reside on the same polypeptide or protein complex. The highest endo-1,4-beta-glucanase-specific activities were observed following DEAE-HPLC chromatography, with 16.2 and 7.5 mumol of glucose equivalents per min per mg of protein for endoglucanases A and B, respectively.(ABSTRACT TRUNCATED AT 250 WORDS)" @default.
• W1567227084 created "2016-06-24" @default.
• W1567227084 creator A5065869693 @default.
• W1567227084 creator A5081579066 @default.
• W1567227084 date "1990-06-01" @default.
• W1567227084 modified "2023-10-17" @default.
• W1567227084 title "Assessment of the endo-1,4-beta-glucanase components of Ruminococcus flavefaciens FD-1" @default.
• W1567227084 cites W143010054 @default.
• W1567227084 cites W1519048429 @default.
• W1567227084 cites W1539161046 @default.
• W1567227084 cites W1547043647 @default.
• W1567227084 cites W1561750927 @default.
• W1567227084 cites W1584488300 @default.
• W1567227084 cites W1613261866 @default.
• W1567227084 cites W1790453471 @default.
• W1567227084 cites W1796756504 @default.
• W1567227084 cites W1822791631 @default.
• W1567227084 cites W1971358178 @default.
• W1567227084 cites W1975717030 @default.
• W1567227084 cites W2004196635 @default.
• W1567227084 cites W2012830962 @default.
• W1567227084 cites W2013334102 @default.
• W1567227084 cites W2014321075 @default.
• W1567227084 cites W2014369142 @default.
• W1567227084 cites W2015759333 @default.
• W1567227084 cites W2027426986 @default.
• W1567227084 cites W2029406796 @default.
• W1567227084 cites W2042997380 @default.
• W1567227084 cites W2044063522 @default.
• W1567227084 cites W2049230219 @default.
• W1567227084 cites W2058514430 @default.
• W1567227084 cites W2064165061 @default.
• W1567227084 cites W2075025771 @default.
• W1567227084 cites W2100837269 @default.
• W1567227084 cites W2102871685 @default.
• W1567227084 cites W2115858464 @default.
• W1567227084 cites W2117838738 @default.
• W1567227084 cites W2123030953 @default.
• W1567227084 cites W2130971509 @default.
• W1567227084 cites W2147572571 @default.
• W1567227084 cites W2165773280 @default.
• W1567227084 cites W2168394697 @default.
• W1567227084 cites W4232534952 @default.
• W1567227084 cites W969808612 @default.
• W1567227084 doi "https://doi.org/10.1128/aem.56.6.1844-1850.1990" @default.
• W1567227084 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/184520" @default.
• W1567227084 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/2383014" @default.
• W1567227084 hasPublicationYear "1990" @default.
• W1567227084 type Work @default.
• W1567227084 sameAs 1567227084 @default.
• W1567227084 citedByCount "47" @default.
• W1567227084 countsByYear W15672270842012 @default.
• W1567227084 countsByYear W15672270842015 @default.
• W1567227084 countsByYear W15672270842016 @default.
• W1567227084 countsByYear W15672270842018 @default.
• W1567227084 countsByYear W15672270842021 @default.
• W1567227084 countsByYear W15672270842022 @default.
• W1567227084 countsByYear W15672270842023 @default.
• W1567227084 crossrefType "journal-article" @default.
• W1567227084 hasAuthorship W1567227084A5065869693 @default.
• W1567227084 hasAuthorship W1567227084A5081579066 @default.
• W1567227084 hasBestOaLocation W15672270841 @default.
• W1567227084 hasConcept C116084860 @default.
• W1567227084 hasConcept C141603559 @default.
• W1567227084 hasConcept C179998833 @default.
• W1567227084 hasConcept C181199279 @default.
• W1567227084 hasConcept C185592680 @default.
• W1567227084 hasConcept C193363816 @default.
• W1567227084 hasConcept C206967267 @default.
• W1567227084 hasConcept C2777914628 @default.
• W1567227084 hasConcept C2779530776 @default.
• W1567227084 hasConcept C43617362 @default.
• W1567227084 hasConcept C55493867 @default.
• W1567227084 hasConceptScore W1567227084C116084860 @default.
• W1567227084 hasConceptScore W1567227084C141603559 @default.
• W1567227084 hasConceptScore W1567227084C179998833 @default.
• W1567227084 hasConceptScore W1567227084C181199279 @default.
• W1567227084 hasConceptScore W1567227084C185592680 @default.
• W1567227084 hasConceptScore W1567227084C193363816 @default.
• W1567227084 hasConceptScore W1567227084C206967267 @default.
• W1567227084 hasConceptScore W1567227084C2777914628 @default.
• W1567227084 hasConceptScore W1567227084C2779530776 @default.
• W1567227084 hasConceptScore W1567227084C43617362 @default.
• W1567227084 hasConceptScore W1567227084C55493867 @default.
• W1567227084 hasIssue "6" @default.
• W1567227084 hasLocation W15672270841 @default.
• W1567227084 hasLocation W15672270842 @default.
• W1567227084 hasLocation W15672270843 @default.
• W1567227084 hasOpenAccess W1567227084 @default.
• W1567227084 hasPrimaryLocation W15672270841 @default.
• W1567227084 hasRelatedWork W1522472272 @default.
• W1567227084 hasRelatedWork W1567227084 @default.
• W1567227084 hasRelatedWork W2006474055 @default.
• W1567227084 hasRelatedWork W2037505132 @default.
• W1567227084 hasRelatedWork W2045151858 @default.
• W1567227084 hasRelatedWork W2366374576 @default.
• W1567227084 hasRelatedWork W2377280289 @default.
• W1567227084 hasRelatedWork W2378290474 @default.

• next