FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1567264882> ?p ?o ?g. }

• W1567264882 endingPage "3221" @default.
• W1567264882 startingPage "3214" @default.
• W1567264882 abstract "Heparin was fractionated by affinity chromatography on immobilized antithrombin III followed by gel filtration on Sephadex G-100. Eighteen fractions were obtained ranging in molecular weight from 9,700 to 34,300 as determined by sedimentation equilibrium. The binding stoichiometries of antithrombin III and thrombin interactions with the heparin of these fractions were measured, using changes in intrinsic and extrinsic fluorescence. Catalytic activity also was measured for each of the heparin fractions. As the molecular weight of heparin varied from about 10,000 to 30,000, the average number of antithrombin and thrombin sites/heparin molecule varied from 1.0 to 2.1 and 2.4 to 6.8. In addition, the molar specific activity increased 5.7-fold, an increase which correlated directly with the product of the number of antithrombin III and thrombin molecules bound. Thus as the number of bound molecules increased with increased molecular weight, the rate of reaction/bound antithrombin III increased in proportion to the number of bound thrombin molecules and vice versa. This can be explained by assuming that heparin functions as a template for both proteins, that all bound thrombin and antithrombin III molecules are accessible to each other, and that the rate at which a bound molecule reacts is proportional to the number of molecules of its interacting counterpart bound. These observations and conclusions are similar to those of Hoylaerts et al. (Hoylaerts, M., Owen, W. G., and Collen, D. (1984) J. Biol. Chem. 259, 5670-5677), who demonstrated that the rate at which single molecules of antithrombin III, covalently attached to heparin, react increases as the thrombin binding capacity (chain length) of heparin increases." @default.
• W1567264882 created "2016-06-24" @default.
• W1567264882 creator A5021282182 @default.
• W1567264882 creator A5064538691 @default.
• W1567264882 creator A5075389112 @default.
• W1567264882 creator A5087753622 @default.
• W1567264882 date "1986-03-01" @default.
• W1567264882 modified "2023-09-26" @default.
• W1567264882 title "Dependence of antithrombin III and thrombin binding stoichiometries and catalytic activity on the molecular weight of affinity-purified heparin." @default.
• W1567264882 cites W1421137972 @default.
• W1567264882 cites W1480767322 @default.
• W1567264882 cites W1483113783 @default.
• W1567264882 cites W1486748187 @default.
• W1567264882 cites W1493150376 @default.
• W1567264882 cites W1518304924 @default.
• W1567264882 cites W1523272172 @default.
• W1567264882 cites W1528764883 @default.
• W1567264882 cites W1561931582 @default.
• W1567264882 cites W1568194294 @default.
• W1567264882 cites W1572725376 @default.
• W1567264882 cites W1574348079 @default.
• W1567264882 cites W1604571455 @default.
• W1567264882 cites W1608119064 @default.
• W1567264882 cites W1651825297 @default.
• W1567264882 cites W1679166880 @default.
• W1567264882 cites W1891400877 @default.
• W1567264882 cites W1919050777 @default.
• W1567264882 cites W1933438817 @default.
• W1567264882 cites W1972999624 @default.
• W1567264882 cites W1973956782 @default.
• W1567264882 cites W1989251486 @default.
• W1567264882 cites W1998573243 @default.
• W1567264882 cites W1999121204 @default.
• W1567264882 cites W1999652145 @default.
• W1567264882 cites W2000111932 @default.
• W1567264882 cites W2004724393 @default.
• W1567264882 cites W2008875799 @default.
• W1567264882 cites W2022778851 @default.
• W1567264882 cites W2038363212 @default.
• W1567264882 cites W2041089953 @default.
• W1567264882 cites W2043404328 @default.
• W1567264882 cites W2049348945 @default.
• W1567264882 cites W2057657597 @default.
• W1567264882 cites W2072600252 @default.
• W1567264882 cites W2075057874 @default.
• W1567264882 cites W2076038286 @default.
• W1567264882 cites W2083066365 @default.
• W1567264882 cites W2086808806 @default.
• W1567264882 cites W2094404759 @default.
• W1567264882 cites W2095224270 @default.
• W1567264882 cites W2113282925 @default.
• W1567264882 cites W2139481768 @default.
• W1567264882 cites W2152939215 @default.
• W1567264882 cites W30895601 @default.
• W1567264882 cites W4249149432 @default.
• W1567264882 cites W68710030 @default.
• W1567264882 cites W84979909 @default.
• W1567264882 doi "https://doi.org/10.1016/s0021-9258(17)35770-8" @default.
• W1567264882 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/3949769" @default.
• W1567264882 hasPublicationYear "1986" @default.
• W1567264882 type Work @default.
• W1567264882 sameAs 1567264882 @default.
• W1567264882 citedByCount "67" @default.
• W1567264882 countsByYear W15672648822013 @default.
• W1567264882 crossrefType "journal-article" @default.
• W1567264882 hasAuthorship W1567264882A5021282182 @default.
• W1567264882 hasAuthorship W1567264882A5064538691 @default.
• W1567264882 hasAuthorship W1567264882A5075389112 @default.
• W1567264882 hasAuthorship W1567264882A5087753622 @default.
• W1567264882 hasBestOaLocation W15672648821 @default.
• W1567264882 hasConcept C126322002 @default.
• W1567264882 hasConcept C144082473 @default.
• W1567264882 hasConcept C161790260 @default.
• W1567264882 hasConcept C178790620 @default.
• W1567264882 hasConcept C185592680 @default.
• W1567264882 hasConcept C2776884760 @default.
• W1567264882 hasConcept C2777292125 @default.
• W1567264882 hasConcept C2777557582 @default.
• W1567264882 hasConcept C2779026020 @default.
• W1567264882 hasConcept C55493867 @default.
• W1567264882 hasConcept C71924100 @default.
• W1567264882 hasConcept C89560881 @default.
• W1567264882 hasConceptScore W1567264882C126322002 @default.
• W1567264882 hasConceptScore W1567264882C144082473 @default.
• W1567264882 hasConceptScore W1567264882C161790260 @default.
• W1567264882 hasConceptScore W1567264882C178790620 @default.
• W1567264882 hasConceptScore W1567264882C185592680 @default.
• W1567264882 hasConceptScore W1567264882C2776884760 @default.
• W1567264882 hasConceptScore W1567264882C2777292125 @default.
• W1567264882 hasConceptScore W1567264882C2777557582 @default.
• W1567264882 hasConceptScore W1567264882C2779026020 @default.
• W1567264882 hasConceptScore W1567264882C55493867 @default.
• W1567264882 hasConceptScore W1567264882C71924100 @default.
• W1567264882 hasConceptScore W1567264882C89560881 @default.
• W1567264882 hasIssue "7" @default.
• W1567264882 hasLocation W15672648821 @default.
• W1567264882 hasOpenAccess W1567264882 @default.
• W1567264882 hasPrimaryLocation W15672648821 @default.

• next