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- W1567391124 abstract "Publisher Summary This chapter discusses the in vivo approaches for studying peptide processing. In vivo approaches are of particular use in determining extracellular processing. Moreover, they are assuming greater importance with the realization that extracellular processing of peptides involves activation as well as inactivation. In vitro studies can be performed in humans, although there may be ethical issues in infusing the peptide. A large animal such as a dog, pig, or sheep is best because it allows multiple blood samples during and after the infusion period. In addition, sufficient blood can be taken to allow chromatography. Ideally the structures and proportions of the peptides in the tissue of origin and in the circulation should be known. Differences in ratios suggest either preferential secretion, differential metabolism, or processing during secretion. These can be distinguished by the combination of clearance studies, organ-specific metabolism, and sampling close to the site of secretion." @default.
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- W1567391124 date "1995-01-01" @default.
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- W1567391124 title "[15] In Vivo Approaches for studying peptide processing" @default.
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- W1567391124 doi "https://doi.org/10.1016/s1043-9471(06)80126-6" @default.
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