FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1567399187> ?p ?o ?g. }

• W1567399187 abstract "Previous research in our laboratory showed that when knockout mutations were created in the pyrB and pyrC genes of the pyrimidine pathway in Pseudomonas aeruginosa, not only were the resultant mutants auxotrophic for pyrimidines but they were also impaired in virulence factor production. Such a correlation had not been previously reported for P. aeruginosa, a ubiquitous opportunistic pathogen in humans. In an earlier study it was reported that mutants blocked in one of the first three enzymes of the pyrimidine pathway in the non-pathogenic strain P. putida M produced no pyoverdin pigment while mutants blocked in the later steps produced copious amounts of pigment, just like the wild type. This study probed for the same connection between pyrimidine auxotrophy and pigment production applied in P. aeruginosa. To that end a knockout mutation was created in pyrD, the fourth step in the pyrimidine pathway which encodes dihydroorotate dehydrogenase. The resulting mutant required pyrimidines for growth but produced wild type pigment levels. Since the pigment pyoverdin is a siderophore it may also be considered a virulence factor, other virulence factors were quantified in the mutant. These included casein protease, hemolysin, elastase, swimming, swarming and twitching motility, and iron binding capacity. In all cases these virulence factors were significantly decreased in the mutant. Even supplementing with uracil did not attain wild type levels. Starvation of the pyrimidine mutant for uracil caused increased specific activity of the pyrimidine enzymes, suggesting that regulation of the pyrimidine pathway occurred at the level of transcription. This effect has also been reported for P. oleovorans. The present research consolidates the idea that pyrimidine auxotrophs cause decreased pathogenicity in P. aeruginosa. Such a finding may open the search for chemotherapy targets in cystic fibrosis and burn victims where P. aeruginosa is an infecting agent." @default.
• W1567399187 created "2016-06-24" @default.
• W1567399187 creator A5022784640 @default.
• W1567399187 date "2005-12-01" @default.
• W1567399187 modified "2023-09-27" @default.
• W1567399187 title "Impaired virulence factor production in a dihydroorotate dehydrogenase mutant (pyrD) of Pseudomonas aeruginosa." @default.
• W1567399187 cites W1293955 @default.
• W1567399187 cites W1508749862 @default.
• W1567399187 cites W1514928730 @default.
• W1567399187 cites W1527200958 @default.
• W1567399187 cites W1568502048 @default.
• W1567399187 cites W1583704529 @default.
• W1567399187 cites W1585869597 @default.
• W1567399187 cites W1594624522 @default.
• W1567399187 cites W1766151574 @default.
• W1567399187 cites W1832566006 @default.
• W1567399187 cites W1842954570 @default.
• W1567399187 cites W1859272595 @default.
• W1567399187 cites W1872991389 @default.
• W1567399187 cites W1903990541 @default.
• W1567399187 cites W1907761738 @default.
• W1567399187 cites W1948277616 @default.
• W1567399187 cites W1965106604 @default.
• W1567399187 cites W1967622238 @default.
• W1567399187 cites W1977105094 @default.
• W1567399187 cites W1978757864 @default.
• W1567399187 cites W1980712809 @default.
• W1567399187 cites W2004020018 @default.
• W1567399187 cites W2008405836 @default.
• W1567399187 cites W2012248059 @default.
• W1567399187 cites W2013740990 @default.
• W1567399187 cites W2014839675 @default.
• W1567399187 cites W2016158447 @default.
• W1567399187 cites W2022033248 @default.
• W1567399187 cites W2025440590 @default.
• W1567399187 cites W2028544641 @default.
• W1567399187 cites W2031582681 @default.
• W1567399187 cites W2035286989 @default.
• W1567399187 cites W2035782757 @default.
• W1567399187 cites W2040774388 @default.
• W1567399187 cites W2050685671 @default.
• W1567399187 cites W2053095874 @default.
• W1567399187 cites W2053213037 @default.
• W1567399187 cites W2064612941 @default.
• W1567399187 cites W2075006858 @default.
• W1567399187 cites W2083233394 @default.
• W1567399187 cites W2086589388 @default.
• W1567399187 cites W2087642068 @default.
• W1567399187 cites W2087679797 @default.
• W1567399187 cites W2099084343 @default.
• W1567399187 cites W2107261335 @default.
• W1567399187 cites W2108074850 @default.
• W1567399187 cites W2112908411 @default.
• W1567399187 cites W2113497222 @default.
• W1567399187 cites W2113771246 @default.
• W1567399187 cites W2118260061 @default.
• W1567399187 cites W2123273294 @default.
• W1567399187 cites W2123890250 @default.
• W1567399187 cites W2124031945 @default.
• W1567399187 cites W2125713628 @default.
• W1567399187 cites W2128578655 @default.
• W1567399187 cites W2128635872 @default.
• W1567399187 cites W2129788869 @default.
• W1567399187 cites W2132941637 @default.
• W1567399187 cites W2138805337 @default.
• W1567399187 cites W2145007319 @default.
• W1567399187 cites W2163182389 @default.
• W1567399187 cites W2169545551 @default.
• W1567399187 cites W2252427397 @default.
• W1567399187 cites W2289232069 @default.
• W1567399187 cites W231863706 @default.
• W1567399187 hasPublicationYear "2005" @default.
• W1567399187 type Work @default.
• W1567399187 sameAs 1567399187 @default.
• W1567399187 citedByCount "1" @default.
• W1567399187 crossrefType "dissertation" @default.
• W1567399187 hasAuthorship W1567399187A5022784640 @default.
• W1567399187 hasConcept C104317684 @default.
• W1567399187 hasConcept C108319773 @default.
• W1567399187 hasConcept C118687296 @default.
• W1567399187 hasConcept C143065580 @default.
• W1567399187 hasConcept C181199279 @default.
• W1567399187 hasConcept C200159599 @default.
• W1567399187 hasConcept C207583985 @default.
• W1567399187 hasConcept C20950039 @default.
• W1567399187 hasConcept C2776476023 @default.
• W1567399187 hasConcept C2777637488 @default.
• W1567399187 hasConcept C2779321679 @default.
• W1567399187 hasConcept C2779554091 @default.
• W1567399187 hasConcept C2779865431 @default.
• W1567399187 hasConcept C2781090273 @default.
• W1567399187 hasConcept C2781427627 @default.
• W1567399187 hasConcept C523546767 @default.
• W1567399187 hasConcept C54355233 @default.
• W1567399187 hasConcept C552990157 @default.
• W1567399187 hasConcept C55493867 @default.
• W1567399187 hasConcept C60987743 @default.
• W1567399187 hasConcept C86803240 @default.
• W1567399187 hasConcept C89423630 @default.
• W1567399187 hasConceptScore W1567399187C104317684 @default.

• next