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- W1567410794 abstract "This units presents methods through which one may isolate and identify novel bacterial DNA-binding proteins. Briefly, the DNA sequence of interest is affixed to beads, and then incubated with bacterial cytoplasmic extract. Washes with buffers containing nonspecific DNA and low-salt concentrations will remove non-adhering and low-specificity DNA-binding proteins, while subsequent washes with higher salt concentrations will elute more specific DNA-binding proteins. Eluted proteins may then be identified by standard proteomic techniques." @default.
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- W1567410794 date "2012-02-01" @default.
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- W1567410794 title "Identification of Novel DNA‐Binding Proteins Using DNA‐Affinity Chromatography/Pull Down" @default.
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- W1567410794 doi "https://doi.org/10.1002/9780471729259.mc01f01s24" @default.
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