Matches in SemOpenAlex for { <https://semopenalex.org/work/W1567416057> ?p ?o ?g. }
Showing items 1 to 86 of
86
with 100 items per page.
- W1567416057 endingPage "300" @default.
- W1567416057 startingPage "300" @default.
- W1567416057 abstract "In vitro fertilization (IVF) and embryonic development of mature and meiotically arrested porcine oocytes were compared in this study. After in vitro maturation (IVM) for 48 h of cumulus-oocyte complexes, 75.4% (n = 442) of them extruded a visible polar body (PB). Most oocytes with a polar body (PB+ group) were found to be at metaphase II (M-II) stage (91.4%). Most oocytes without a visible polar body (PB− group, n = 144) appeared to be arrested at the germinal vesicle (GV) (41.6%) and first meiotic metaphase (M-I) (34.0%) stages. After IVF of oocytes (the day of IVF = Day 0), there was no significant difference between PB+ and PB− groups in rates of sperm penetration, monospermy, and oocyte activation after the penetration. Embryonic development was assessed by staining with 1% orcein. On Day 2, although there was no difference between the embryo cleavage in PB+ (n = 447) and PB− (n = 217) groups (47.0% and 35.9%, respectively), PB+ embryos had more cells than the PB− embryos (3.37 and 2.81 cells, respectively) (P < 0.05; ANOVA). On Day 4, the cleavage rate of PB+ embryos was higher than that of PB− embryos (45.4% and 24.3%, respectively), and PB+ embryos had more cells than the PB− embryos (8.26 and 6.0 cells, respectively) (P < 0.05; ANOVA). On Day 6, a significantly higher number of PB+ embryos developed to the blastocyst stage than that of the PB− embryos (34.6% and 20.7%, respectively) (P < 0.05). However, by subtracting the GV oocytes from the PB− group, there was no difference in blastocyst rates between the M-I arrested and M-II oocytes (35.3% and 34.6%, respectively). The number of blastomer nuclei in embryos obtained from the PB+ group (52.0) was significantly higher than that of the PB− group (29.1); however, the proportion of inner cell mass and trophectoderm cells in PB+ and PB− blastocysts did not differ significantly (1:1.9 and 1:2.2, respectively) (P < 0.05). Chromosome analysis revealed that PB+ blastocysts had significantly more diploid blastomeres (69.7%) than PB− blastocysts (44.0%), whereas PB− blastocysts had significantly more triploid cells (34.0%) compared with PB+ oocytes (8.4%)(P < 0.05; χ2 test). These results indicate that porcine oocytes arrested at the M-I stage undergo cytoplasmic maturation during culture and have the same ability to develop to blastocysts after IVF as M-II oocytes but with a lower cell number; the latter might be caused by the slower embryonic development." @default.
- W1567416057 created "2016-06-24" @default.
- W1567416057 creator A5005193322 @default.
- W1567416057 creator A5009980087 @default.
- W1567416057 creator A5019934818 @default.
- W1567416057 creator A5026651411 @default.
- W1567416057 creator A5030885340 @default.
- W1567416057 creator A5035502126 @default.
- W1567416057 creator A5049285971 @default.
- W1567416057 creator A5053482370 @default.
- W1567416057 creator A5071927184 @default.
- W1567416057 creator A5074354992 @default.
- W1567416057 creator A5079584496 @default.
- W1567416057 creator A5090489629 @default.
- W1567416057 date "2005-01-01" @default.
- W1567416057 modified "2023-10-17" @default.
- W1567416057 title "299 IN VITRO DEVELOPMENT OF IMMATURE PORCINE OOCYTES FERTILIZED IN VITRO TO THE BLASTOCYST STAGE" @default.
- W1567416057 doi "https://doi.org/10.1071/rdv17n2ab299" @default.
- W1567416057 hasPublicationYear "2005" @default.
- W1567416057 type Work @default.
- W1567416057 sameAs 1567416057 @default.
- W1567416057 citedByCount "1" @default.
- W1567416057 countsByYear W15674160572023 @default.
- W1567416057 crossrefType "journal-article" @default.
- W1567416057 hasAuthorship W1567416057A5005193322 @default.
- W1567416057 hasAuthorship W1567416057A5009980087 @default.
- W1567416057 hasAuthorship W1567416057A5019934818 @default.
- W1567416057 hasAuthorship W1567416057A5026651411 @default.
- W1567416057 hasAuthorship W1567416057A5030885340 @default.
- W1567416057 hasAuthorship W1567416057A5035502126 @default.
- W1567416057 hasAuthorship W1567416057A5049285971 @default.
- W1567416057 hasAuthorship W1567416057A5053482370 @default.
- W1567416057 hasAuthorship W1567416057A5071927184 @default.
- W1567416057 hasAuthorship W1567416057A5074354992 @default.
- W1567416057 hasAuthorship W1567416057A5079584496 @default.
- W1567416057 hasAuthorship W1567416057A5090489629 @default.
- W1567416057 hasBestOaLocation W15674160571 @default.
- W1567416057 hasConcept C105702510 @default.
- W1567416057 hasConcept C16685009 @default.
- W1567416057 hasConcept C185592680 @default.
- W1567416057 hasConcept C196843134 @default.
- W1567416057 hasConcept C201604863 @default.
- W1567416057 hasConcept C2775966511 @default.
- W1567416057 hasConcept C2776690073 @default.
- W1567416057 hasConcept C2778177303 @default.
- W1567416057 hasConcept C2994119143 @default.
- W1567416057 hasConcept C54355233 @default.
- W1567416057 hasConcept C71924100 @default.
- W1567416057 hasConcept C86803240 @default.
- W1567416057 hasConcept C87073359 @default.
- W1567416057 hasConcept C88972607 @default.
- W1567416057 hasConceptScore W1567416057C105702510 @default.
- W1567416057 hasConceptScore W1567416057C16685009 @default.
- W1567416057 hasConceptScore W1567416057C185592680 @default.
- W1567416057 hasConceptScore W1567416057C196843134 @default.
- W1567416057 hasConceptScore W1567416057C201604863 @default.
- W1567416057 hasConceptScore W1567416057C2775966511 @default.
- W1567416057 hasConceptScore W1567416057C2776690073 @default.
- W1567416057 hasConceptScore W1567416057C2778177303 @default.
- W1567416057 hasConceptScore W1567416057C2994119143 @default.
- W1567416057 hasConceptScore W1567416057C54355233 @default.
- W1567416057 hasConceptScore W1567416057C71924100 @default.
- W1567416057 hasConceptScore W1567416057C86803240 @default.
- W1567416057 hasConceptScore W1567416057C87073359 @default.
- W1567416057 hasConceptScore W1567416057C88972607 @default.
- W1567416057 hasIssue "2" @default.
- W1567416057 hasLocation W15674160571 @default.
- W1567416057 hasOpenAccess W1567416057 @default.
- W1567416057 hasPrimaryLocation W15674160571 @default.
- W1567416057 hasRelatedWork W1567416057 @default.
- W1567416057 hasRelatedWork W1975321368 @default.
- W1567416057 hasRelatedWork W2066157877 @default.
- W1567416057 hasRelatedWork W2082215944 @default.
- W1567416057 hasRelatedWork W2166971475 @default.
- W1567416057 hasRelatedWork W2244308131 @default.
- W1567416057 hasRelatedWork W2349296746 @default.
- W1567416057 hasRelatedWork W2389182588 @default.
- W1567416057 hasRelatedWork W2589363550 @default.
- W1567416057 hasRelatedWork W2991142203 @default.
- W1567416057 hasVolume "17" @default.
- W1567416057 isParatext "false" @default.
- W1567416057 isRetracted "false" @default.
- W1567416057 magId "1567416057" @default.
- W1567416057 workType "article" @default.