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- W1567440243 abstract "Publisher Summary This chapter discusses the measurement of immune responses. When analyzing the immune response in experimentally infected animals, the goal is both to detect differences in the kinetics and magnitude of, for instance, cytokine expression in individual tissues and to define the cellular localization of this response. Cross-contamination is the biggest problem in all PCR procedures and great care should be taken to avoid the contamination of test samples with extraneous sources of DNA. For primer selection, primers should be complementary to sequences in separate exons, or should span exon–exon boundaries, to be mRNA/cDNA specific. The purpose of semiquantitative PCR is to compare RNA samples from different sources for their relative content of specific mRNAs. During the PCR, a constant amount of the control DNA is added to the PCR mixture. The correct amount will depend on the amount of amplifiable cDNA in the sample and if a lot of specific cDNA can be amplified, only very little competitor needs to be added." @default.
- W1567440243 created "2016-06-24" @default.
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- W1567440243 date "1998-01-01" @default.
- W1567440243 modified "2023-09-27" @default.
- W1567440243 title "3.2 Measuring Immune Responses" @default.
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- W1567440243 doi "https://doi.org/10.1016/s0580-9517(08)70686-x" @default.
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