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- W1567445289 abstract "Two fatty acid desaturases were independently expressed in tobacco plants and freezing tolerance of the transgenic plants was investigated: the two desaturases were CVFAD2, which desaturates microsomal C18:1 (oleic acid) to C18:2 (linoleic acid), and CVFAD3, which desaturates microsomal C18:2 to C18:3 (linolenic acid). Fatty acid composition analysis showed that the C18:2 contents in the plants transforrned with pBE21131CvFad2 did not increase compared with that of the wlld-type plant. However, an increase in the C18:3 content by 2.40/0 was observed in the line of No. 19 although the increase was not statistically significant. In the plant transformed with pBE21131CvFad3, the C18:3 content increased by 6.40/0 compared with that of the wlld-type plant. Measurement of the electrolyte leakage of the leaves showed that freezing tolerance of the CvFad2 plant was a little higher than that of the wild-type plant at all temperatures investigated (from -1 to -4 'C). Contrary to the case of the CvFad2, the freezing tolerance of the CvFad3 plant tended to be sllghtly lower than that of the wild-type at all temperatures tested despite the unsaturation levels of the plant were higher than those of the CvFad2 plants." @default.
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- W1567445289 date "2003-02-01" @default.
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- W1567445289 title "Freezing Tolerance of Transgenic Tobacco with Increased Content of Unsaturated Fatty Acid by Expressiong the CvFad3 Gene" @default.
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- W1567445289 doi "https://doi.org/10.5109/4500" @default.
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