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- W1567449622 abstract "A highly improved procedure for the preparation of ecdysone-protein conjugates for immunological work is reported. Bovine thyroglobulin is succinylated and the succinylated protein is coupled to beta-ecdysone with 1-ethyl-3-(-dimethylaminopropyl)-carbodiimide in the presence of the acylation catalyst 4-dimethylaminopyridine, The antiserum obtained using this immunogen provides a radioimmunoassay sensitive to 25 pmol of beta-ecdysone. The anti-ecdysone antibody cross-reacts with alpha-ecdysone but not with cholesterol or progesterone. This procedure reverses the standard strategy for synthesizing ester linkages in hapten-protein conjugates and should have widespread applicability for the preparation of other such conjugates for immunological work." @default.
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- W1567449622 date "1980-08-01" @default.
- W1567449622 modified "2023-09-30" @default.
- W1567449622 title "Preparation of an ecdysone immunogen for radioimmunoassay work." @default.
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- W1567449622 doi "https://doi.org/10.1016/s0021-9258(18)43698-8" @default.
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